Direct particle-to-cell deposition of coarse ambient particulate matter increases the production of inflammatory mediators from cultured human airway epithelial cells.
Direct particle-to-cell deposition of coarse ambient particulate matter increases the production of inflammatory mediators from cultured human airway epithelial cells.
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DOI：
10.1021/es900698a
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发表时间：
2009-06-15
影响因子：
11.4
通讯作者：
Devlin, Robert B.
中科院分区：
文献类型：
--
作者：
Volckens, John;Dailey, Lisa;Walters, Glenn;Devlin, Robert B.
培养的细胞暴露于颗粒物空气污染通常通过以下方式完成：在固体基质上收集颗粒，从基质中提取颗粒，将它们悬浮在液体中，并将悬浮液应用于在塑料上生长并浸没在培养基中的细胞。这项工作的目的是开发一个更生理和环境相关的模型，空气污染物沉积在培养的人原代气道上皮细胞。我们假设吸入颗粒物的毒理学强烈依赖于颗粒分散状态和传递到细胞的模式。我们的暴露系统采用单极充电和静电力的组合，以将颗粒直接从空气中存款到在加热、加湿的暴露室中的气液界面处生长的细胞上。在该系统中，暴露于浓缩的粗糙环境颗粒物的正常人支气管上皮细胞在暴露后1小时和相对于暴露于无颗粒空气的对照组表达增加的炎症生物标志物水平。更重要的是，在比使用传统体外系统施加的水平低1-2个数量级的颗粒负载下观察到这些效应。
Exposure of cultured cells to particulate matter air pollution is usually accomplished by collecting particles on a solid matrix, extracting the particles from the matrix, suspending them in liquid, and applying the suspension to cells grown on plastic and submerged in medium. The objective of this work was to develop a more physiologically and environmentally relevant model of air pollutant deposition on cultures of human primary airway epithelial cells. We hypothesize that the toxicology of inhaled particulate matter depends strongly on both the particulate dispersion state and the mode of delivery to cells. Our exposure system employs a combination of unipolar charging and electrostatic force to deposit particles directly from the air onto cells grown at an air-liquid interface in a heated, humidified exposure chamber. Normal human bronchial epithelial cells exposed to concentrated, coarse ambient particulate matter in this system expressed increased levels of inflammatory biomarkers at 1 hour following exposure and relative to controls exposed to particle-free air. More importantly, these effects are seen at particulate loadings that are 1-2 orders of magnitude lower than levels applied using traditional in vitro systems.
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影响因子：
5.2
作者：
VANDERPOOL, RW;RUBOW, KL
通讯作者：
RUBOW, KL
DOI：
10.1165/rcmb.2006-0466oc
发表时间：
2007-08-01
影响因子：
6.4
作者：
Ross, Andrea J.;Dailey, Lisa A.;Devlin, Robert B.
通讯作者：
Devlin, Robert B.
影响因子：
2.7
作者：
Diabate, Silvia;Muelhopt, Sonja;Krug, Harald F.
通讯作者：
Krug, Harald F.
影响因子：
--
作者：
Aufderheide, M;Mohr, U
通讯作者：
Mohr, U
影响因子：
10.4
作者：
Xia T;Korge P;Weiss JN;Li N;Venkatesen MI;Sioutas C;Nel A
通讯作者：
Nel A