Abscisic acid enhances tolerance of wheat seedlings to drought and regulates transcript levels of genes encoding ascorbate-glutathione biosynthesis.
Abscisic acid enhances tolerance of wheat seedlings to drought and regulates transcript levels of genes encoding ascorbate-glutathione biosynthesis.
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DOI：
10.3389/fpls.2015.00458
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发表时间：
2015
影响因子：
5.6
通讯作者：
Kang G
中科院分区：
文献类型：
--
作者：
Wei L;Wang L;Yang Y;Wang P;Guo T;Kang G
谷胱甘肽（GSH）和抗坏血酸（ASA）与脱落酸（ABA）诱导的高等植物非生物耐受性有关，但其分子机制尚不清楚。在本研究中，外源添加（10 μM） ABA显著提高了普通小麦（Triticum aestivum L.）幼苗在5 d 15%聚乙二醇（PEG）刺激下的耐旱性，表现为茎长、茎和根干重增加，过氧化氢（H2O2）和丙二醛（MDA）含量降低。干旱胁迫下，ABA显著提高了植物叶片和根系中GSH和ASA的含量。采用实时定量反转录聚合酶链反应（qPCR）检测ASA和GSH合成相关酶的8个基因的时空表达模式。结果表明，ABA可暂时调控ASA-GSH循环酶编码基因的转录水平。干旱胁迫下，这些基因在aba处理的小麦幼苗的根和叶器官中表现出不同的表达模式。这些结果表明，外源ABA增加了干旱胁迫小麦幼苗GSH和ASA水平，并具有时间特异性和器官特异性。此外，我们比较了ABA和水杨酸（SA）处理小麦幼苗在peg刺激干旱胁迫下叶片组织中ASA-GSH合成相关酶基因的转录谱，表明它们通过差异调节ASA-GSH合成酶基因的表达水平来提高ASA和GSH的含量。我们的研究结果增加了我们对aba诱导高等植物抗旱性的分子机制的理解。
Glutathione (GSH) and ascorbate (ASA) are associated with the abscisic acid (ABA)-induced abiotic tolerance in higher plant, however, its molecular mechanism remains obscure. In this study, exogenous application (10 μM) of ABA significantly increased the tolerance of seedlings of common wheat (Triticum aestivum L.) suffering from 5 days of 15% polyethylene glycol (PEG)-stimulated drought stress, as demonstrated by increased shoot lengths and shoot and root dry weights, while showing decreased content of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Under drought stress conditions, ABA markedly increased content of GSH and ASA in both leaves and roots of ABA-treated plants. Temporal and spatial expression patterns of eight genes encoding ASA and GSH synthesis-related enzymes were measured using quantitative real-time reverse transcription polymerase chain reaction (qPCR). The results showed that ABA temporally regulated the transcript levels of genes encoding ASA-GSH cycle enzymes. Moreover, these genes exhibited differential expression patterns between the root and leaf organs of ABA-treated wheat seedlings during drought stress. These results implied that exogenous ABA increased the levels of GSH and ASA in drought-stressed wheat seedlings in time- and organ-specific manners. Moreover, the transcriptional profiles of ASA-GSH synthesis-related enzyme genes in the leaf tissue were compared between ABA- and salicylic acid (SA)-treated wheat seedlings under PEG-stimulated drought stress, suggesting that they increased the content of ASA and GSH by differentially regulating expression levels of ASA-GSH synthesis enzyme genes. Our results increase our understanding of the molecular mechanism of ABA-induced drought tolerance in higher plants.
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影响因子：
11.4
作者：
Chinnusamy V;Gong Z;Zhu JK
通讯作者：
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影响因子：
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5.7
作者：
Li, Yan;Zhao, Hongxia;Li, Chunyang
通讯作者：
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影响因子：
6.4
作者：
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