Reference gene selection in the desert plant Eremosparton songoricum.
Reference gene selection in the desert plant Eremosparton songoricum.
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DOI：
10.3390/ijms13066944
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发表时间：
2012
影响因子：
5.6
通讯作者：
Wood AJ
中科院分区：
文献类型：
--
作者：
Li XS;Yang HL;Zhang DY;Zhang YM;Wood AJ
准噶尔无叶草Eremosparton songoricum（Litv.）瓦斯（E. songoricum）是一种罕见的和极其耐旱的沙漠植物，有望作为鉴定与缺水胁迫相关的基因的模式生物。在这里，我们克隆和评估的八个候选参考基因的表达，使用定量实时逆转录聚合酶链反应。这些候选参考基因的表达在包括各种大肠杆菌在内的20个样品的不同集合中进行了分析。songoricum植物组织暴露于多种环境胁迫。GeNorm分析表明，在不同的实验条件下，参考基因之间的表达稳定性不同，但在大多数条件下，两个最稳定的参考基因足以进行归一化。EsEF和Esα-TUB对各种胁迫条件都是足够的，EsEF和EsACT适用于不同萌发阶段的样品，EsGAPDH和EsUBQ在多个成体组织样品中最稳定。Es 18 S基因不适合作为我们分析的参考基因。此外，干旱胁迫相关转录因子EsDREB 2的表达水平验证了E. songoricum参考基因的结果，并表明没有一个基因本身足以进行标准化。本文首次系统报道了E. songoricum，这些数据将有助于在这个物种的基因表达的未来工作。
Eremosparton songoricum (Litv.) Vass. (E. songoricum) is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various E. songoricum plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions. EsEF and Esα-TUB were sufficient for various stress conditions, EsEF and EsACT were suitable for samples of differing germination stages, and EsGAPDHand EsUBQ were most stable across multiple adult tissue samples. The Es18S gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor EsDREB2 verified the utility of E. songoricum reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in E. songoricum, and these data will facilitate future work on gene expression in this species.
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影响因子：
5.3
作者：
Artico S;Nardeli SM;Brilhante O;Grossi-de-Sa MF;Alves-Ferreira M
通讯作者：
Alves-Ferreira M
影响因子：
1.8
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影响因子：
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作者：
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胡杨 DREB2 型基因的表达谱和功能表征
DOI：
10.1016/j.bbrc.2008.11.071
发表时间：
2009-01-16
影响因子：
3.1
作者：
Chen, Jinhuan;Xia, Xinli;Yin, Weilun
通讯作者：
Yin, Weilun
DOI：
10.1016/j.bbrc.2010.04.079
发表时间：
2010-05-28
影响因子：
3.1
作者：
Garg, Rohini;Sahoo, Annapurna;Jain, Mukesh
通讯作者：
Jain, Mukesh