Translation of the placenta growth factor mRNA is severely affected by a small open reading frame localized in the 5' untranslated region.
Translation of the placenta growth factor mRNA is severely affected by a small open reading frame localized in the 5' untranslated region.
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胎盘生长因子 mRNA 的翻译受到位于 5 非翻译区的小开放阅读框的严重影响。
作者：
D. Maglione;Valente Guerriero;Marco Rambaldi;Giuseppe Russo;M. Graziella Persico
最近发现的胎盘生长因子基因（PlGF）编码与血管通透性因子（VPF）相关的蛋白质。我们提出的证据表明，该基因的表达可以在转录后水平进行调控。PlGF mRNA编码区上游区域含有一个小的开放阅读框（ORF），可能编码15个氨基酸的肽。不同构建体在网织红细胞和麦胚裂解物以及COS-1和CV-1细胞中的翻译表明，该短区域是翻译抑制元件，因为其两个潜在起始密码子中的突变增加体内PIGF合成。利用RNA酶保护实验，我们证明从人足月胎盘和JEG绒毛膜癌细胞系中获得的PIGF mRNA具有完整的5'非翻译区，因此也具有上述小ORF。最后，牛PIGF基因组克隆的分析表明，这个小的ORF相对于推定的肽序列和与PIGF编码区的距离是高度保守的。
The recently identified placenta growth factor gene (PIGF) code for a protein related to the vascular permeability factor (VPF). We present evidence indicating that expressing of this gene could be regulated at the post-transcriptional level. The region upstream to the coding region of PIGF mRNA contains a small open reading frame (ORF), potentially coding for a peptide of 15 amino acids. The translation of different constructs in reticulocyte and wheat germ lysates as well as in COS-1 and CV-1 cells indicates that this short region is a translational inhibitory element since mutations in its two potential initiator codons increase PIGF synthesis in vivo. Using RNAse protection assay, we demonstrate that the PIGF mRNAs obtained from human term placenta and JEG choriocarcinoma cell line have a complete 5' untranslated region and, consequently, also the above mentioned small ORF. Finally, the analysis of a bovine PIGF genomic clone reveals that this small ORF is strongly conserved with respect to both putative peptide sequences and distance from the PIGF coding region.
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影响因子：
11.2
作者：
I. Fidler
通讯作者：
I. Fidler
人类 c-sis 基因 5 部分的序列：转录启动子的表征以及 5 非翻译 mRNA 序列对蛋白质产物表达的调节。
影响因子：
14.9
作者：
Ratner,L;Thielan,B;Collins,T
通讯作者：
Collins,T
影响因子：
5.3
作者：
KOZAK, M
通讯作者：
KOZAK, M
DOI：
10.1016/s0021-9258(19)34036-0
发表时间：
1990-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Elizabeth C. Theil
通讯作者：
Elizabeth C. Theil
DOI：
10.1073/pnas.83.9.2850
发表时间：
1986-05-01
影响因子：
11.1
作者：
KOZAK, M
通讯作者：
KOZAK, M