Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine.
Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine.
复制标题
拥挤的粘虫中免疫力的改变是由章鱼胺和多巴胺介导的
DOI：
10.1038/s41598-018-20711-8
复制
发表时间：
2018-02-16
影响因子：
4.6
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Kong H;Dong C;Tian Z;Mao N;Wang C;Cheng Y;Zhang L;Jiang X;Luo L
与病原性感染类似，高种群密度会改变昆虫的预防性免疫。在许多多酚昆虫中已经报道了密度依赖的预防，但这种现象背后的调控机制尚不清楚。生物源单胺类化合物在调节昆虫免疫反应中发挥着重要作用。在本研究中，研究了粘虫多酚幼虫在1、2、5、10和30只/650mLJAR的密度下的免疫能力和三种生物源单胺的水平。在高密度(5、10、30尾/缸)幼虫体内酚氧化酶(PO)活性和血细胞总数增加的同时，章鱼体内的章鱼胺水平也随之升高。相反，多巴胺水平下降，5-羟色胺水平没有明显影响。注射章鱼胺可引起小鼠血细胞总数和PO活性显著升高。反之，章鱼胺的特异性拮抗剂依匹斯汀可降低血细胞总数和PO活性。另一种章鱼胺拮抗剂酚妥拉明可抑制PO和溶菌酶的活性。此外，注射多巴胺还能显著提高PO活性，降低血细胞总数和溶菌酶活性。这些结果表明，章鱼和多巴胺都介导了拥挤幼虫血细胞总数和PO活性的增加。
Similar to pathogenic infection, high population density alters insects’ prophylactic immunity. Density-dependent prophylaxis has been reported in many polyphenic insects, but the regulatory mechanism underlying this phenomenon remains unclear. The biogenic monoamines are known to play critical roles in mediating insect immune responses. In the current study, the immune capacity and the levels of three biogenic monoamines were investigated in the polyphenic larvae of Mythimna separata, reared at the densities of 1, 2, 5, 10, and 30 larvae per 650-mL jar. Concomitant with the increased phenoloxidase (PO) activity and total haemocyte count in the larvae at high densities (5, 10, 30 larvae/jar), the octopamine level was also increased. In contrast, the dopamine level was decreased, and the 5-hydroxytryptamine level was not significantly affected. Injection of octopamine induced significant increases in the total haemocyte count and PO activity. Conversely, epinastine, a specific antagonist of octopamine, decreased the total haemocyte count and PO activity. Another octopamine antagonist, phentolamine, inhibited the activity of PO and lysozymes. In addition, injection of dopamine induced a significant increase in PO activity and decreased the total haemocyte count and lysozyme activity. These results suggested that both octopamine and dopamine mediate the increases in total haemocyte count and PO activity in the crowded larvae.
登录
查看更多内容
影响因子：
1.3
作者：
KUNIMI, Y;YAMADA, E
通讯作者：
YAMADA, E
影响因子：
15.1
作者：
Adamo, S. A.
通讯作者：
Adamo, S. A.
影响因子：
2.5
作者：
Adamo, S. A.;Parsons, N. M.
通讯作者：
Parsons, N. M.
DOI：
10.1007/s00360-015-0953-1
发表时间：
2016-02-01
影响因子：
2
作者：
Brent, Colin S.;Miyasaki, Katelyn;Nath, Rachna
通讯作者：
Nath, Rachna
影响因子：
0.9
作者：
Iba, M;Nagao, T;Urano, A
通讯作者：
Urano, A