Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation.
Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation.
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DOI：
10.1016/j.jamcollsurg.2011.12.032
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发表时间：
2012-04
影响因子：
5.2
通讯作者：
Pritts, Timothy A.
中科院分区：
文献类型：
--
作者：
Belizaire, Ritha M.;Prakash, Priya S.;Richter, Jillian R.;Robinson, Bryce R.;Edwards, Michael J.;Caldwell, Charles C.;Lentsch, Alex B.;Pritts, Timothy A.
输入储存的血液会增加并发症。微颗粒（MPs）是红细胞释放的小囊泡，可诱导细胞功能障碍，但红细胞来源的MPs在失血性休克复苏中的作用尚不清楚。在目前的研究中，我们研究了红细胞来源的MPs对宿主出血和复苏反应的影响。从小鼠红细胞中分离MPs，用流式细胞术定量，注射到健康小鼠体内。不同的小鼠组分别进行出血和复苏，有或没有红细胞来源的MPs。采集肺进行组织学和中性粒细胞积累，并通过髓过氧化物酶含量进行评估。用人红细胞来源的MPs和CD11b处理人中性粒细胞，测定其表达、超氧化物产生和吞噬活性。与新鲜单位相比，储存的小鼠红细胞单位含有更多的红细胞衍生MPs。与未使用MPs复苏的小鼠相比，使用MPs复苏的出血性小鼠表现出明显增加的肺中性粒细胞积累和肺组织学改变。正常小鼠静脉注射MPs导致中性粒细胞启动，中性粒细胞CD11b表达增加。与未经处理的中性粒细胞相比，经红细胞来源的MPs处理的人中性粒细胞表现出CD11b表达增加、超氧化物产生增加和吞噬能力增强。储存的包装红细胞单位含有红细胞衍生MPs的数量增加。这些MPs似乎有助于中性粒细胞的启动和激活。储存单位中MPs的存在可能与输血后的不良反应有关，包括肺损伤。
Transfusion of stored blood is associated with increased complications. Microparticles (MPs) are small vesicles released from RBCs that can induce cellular dysfunction, but the role of RBC-derived MPs in resuscitation from hemorrhagic shock is unknown. In the current study, we examined the effects of RBC-derived MPs on the host response to hemorrhage and resuscitation. MPs were isolated from murine packed RBC units, quantified using flow cytometry, and injected into healthy mice. Separate groups of mice underwent hemorrhage and resuscitation with and without packed RBC–derived MPs. Lungs were harvested for histology and neutrophil accumulation and assessed by myeloperoxidase content. Human neutrophils were treated with human RBC-derived MPs and CD11b expression, superoxide production, and phagocytic activity were determined. Stored murine packed RBC units contained increased numbers of RBC-derived MPs compared with fresh units. Hemorrhaged mice resuscitated with MPs demonstrated substantially increased pulmonary neutrophil accumulation and altered lung histology compared with mice resuscitated without MPs. Intravenous injection of MPs into normal mice resulted in neutrophil priming, evidenced by increased neutrophil CD11b expression. Human neutrophils treated with RBC-derived MPs demonstrated increased CD11b expression, increased superoxide production, and enanced phagocytic ability compared with untreated neutrophils. Stored packed RBC units contain increased numbers of RBC-derived MPs. These MPs appear to contribute to neutrophil priming and activation. The presence of MPs in stored units can be associated with adverse effects, including lung injury, after transfusion.
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影响因子：
20.3
作者：
Schnabel, Renate B.;Baumert, Jens;Tracy, Russell P.
通讯作者：
Tracy, Russell P.
影响因子：
20.3
作者：
Mangalmurti, Nilam S.;Xiong, Zeyu;Lee, Janet S.
通讯作者：
Lee, Janet S.
影响因子：
4.9
作者：
Labro, MT;Ollivier, V;Babin-Chevaye, C
通讯作者：
Babin-Chevaye, C
影响因子：
3.7
作者：
Sparrow, Rosemary L.
通讯作者：
Sparrow, Rosemary L.
红细胞微粒显示出改变的炎症趋化因子结合并在与血小板相互作用时释放配体。
影响因子：
2.9
作者：
Xiong Z;Cavaretta J;Qu L;Stolz DB;Triulzi D;Lee JS
通讯作者：
Lee JS