Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms.
Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms.
复制标题
辣椒素对 NADH-醌氧化还原酶的抑制与多种生物体中能量偶联位点 1 的存在相关。
DOI：
10.1016/0003-9861(90)90448-8
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发表时间：
1990
影响因子：
3.9
通讯作者：
Yagi,T
中科院分区：
文献类型：
--
作者：
Yagi,T
辣椒素和二氢辣椒素抑制了几种生物呼吸链的NADH-泛醌还原酶活性，这两种辣椒素是红辣椒的辛辣成分。这种抑制与呼吸链的这一段中存在一个能量转导部位有关。当NADH-苯醌氧化还原酶片段涉及能量偶联位点(例如，在脱氮副球藻、大肠杆菌和嗜热嗜热菌HB-8膜和牛心脏线粒体中)时，辣椒素起到抑制NADH还原泛醌的作用。相反，在没有这个能量偶联位点的地方(例如，在酿酒酵母线粒体和枯草芽孢杆菌膜中)，辣椒素对NADH-泛醌还原酶活性没有抑制作用。用含牛血清白蛋白的培养基洗膜，可逆转辣椒素对膜的抑制作用。在你身上。在结肠、副触角膜和牛亚线粒体颗粒中，辣椒素在较低浓度的泛醌-1(<；20μm)时对泛醌-1起非竞争性抑制作用，在较高浓度的泛醌-1(>；50μm)时起竞争抑制作用。此外，在牛亚线粒体颗粒中加入辅酶Q-10后，抑制NADH酶活性50%所需的辣椒素浓度增加。本文还讨论了辣椒素抑制能量转导的NADH-Q氧化还原酶的机制。
The NADH-ubiquinone reductase activity of the respiratory chains of several organisms was inhibited by capsaicin and dihydrocapsaicin, which are the pungent principles of red pepper. This inhibition was correlated with the presence of an energy transducing site in this segment of the respiratory chain. Where the NADH-quinone oxidoreductase segment involved an energy coupling site (e.g., inParacoccus denitrificans, Escherichia coli, andThermus thermophilusHB-8 membranes and bovine heart mitochondria), capsaicin acted as an inhibitor of ubiquinone reduction by NADH. In contrast, where this energy coupling site was absent (e.g., inSaccharomyces cerevisiaemitochondria andBacillus subtilismembranes), there was no inhibition of NADH-ubiquinone reductase activity by capsaicin. The capsaicin inhibition ofParacoccusmembranes was reversed by washing the membranes with medium containing bovine serum albumin. In theE. coliandParacoccusmembranes and bovine submitochondrial particles, capsaicin acted as a noncompetitive inhibitor for ubiquinone-1 at lower concentrations of ubiquinone-1 (<20 μm) and as a competitive inhibitor at higher concentrations of ubiquinone-1 (>50 μm). In addition, the concentrations of capsaicin required for 50% inhibition of NADH oxidase activity of bovine submitochondrial particles were increased when ubiquinone-10 was added to the particles. The mechanism by which capsaicin inhibits the energy-transducing NADH-quinone oxidoreductase is discussed.
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