Automated latex photometric immunoassay for total plasminogen activator inhibitor-1 in plasma.
Automated latex photometric immunoassay for total plasminogen activator inhibitor-1 in plasma.
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血浆中总纤溶酶原激活剂抑制剂 1 的自动乳胶光度免疫分析。
DOI：
10.1373/49.6.987
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发表时间：
2003
影响因子：
9.3
通讯作者：
F. Furusaki
中科院分区：
文献类型：
--
作者：
Tomoko Ono;Maki Sogabe;M. Ogura;F. Furusaki
纤溶酶原激活剂抑制剂-1 (PAI-1) 是纤溶系统的关键调节剂 (1)。持续较高的 PAI-1 浓度使纤维蛋白难以被组织型纤溶酶原激活剂 (tPA) 溶解，从而导致患者多器官功能障碍和不良预后 (2)。已经开发了几种测量血浆样品中 PAI-1 功能活性的方法，其原理是添加超过 PAI-1 的指定量的 tPA，并在短的潜伏期后测量残留的 tPA 活性 (3)。然而，这些方法既不完全特异性也不准确，并且血浆中的许多其他蛋白酶抑制剂会抑制 tPA (4)。尽管也描述了基于使用单克隆和/或多克隆抗体的两步酶免疫测定的 PAI-1 抗原的几种测定法 (5)(6)，但它们的测量范围较窄 (0–40 μg/L) 并且非常耗时 (7)。由于 PAI-1 是一种不稳定分子，因此在血液采集和样品处理过程中需要格外小心，以确保准确测量 PAI-1 和 tPA。 PAI-1 从活性形式转化为无活性潜伏形式的半衰期在体外约为 4 小时，在体内甚至更短 (8)。因此，必须正确评估内皮细胞和脂肪组织释放的 PAI-1 量 (9)，以使此类方法在临床上有用。我们开发了一种在动态测量范围内检测总 PAI-1 的乳胶光度免疫分析 (LPIA)，可以检测所有形式的 PAI-1，而不受构象变化的影响。
样本取自 47 名心肌梗死患者（29 名男性和 18 名女性）和 276 名自愿参加本研究的医院员工（作为对照）[100 名男性（年龄范围，23-63 岁）和 168 名女性（年龄范围，21-57 岁）]。知情同意后，将血样采集到 1/10 体积的含有 38 g/L 钠的溶液中……
Plasminogen activator inhibitor-1 (PAI-1) is a key regulator of the fibrinolytic system (1). The continuously high PAI-1 concentrations make fibrins resistant to dissolution by tissue-type plasminogen activator (tPA), which leads to multiorgan dysfunction and a bad prognosis in patients (2). Several methods have been developed for measuring the functional activity of PAI-1 in plasma samples based on the principle of adding a specified amount of tPA in excess of the PAI-1 and measuring residual tPA activity after a short incubation period (3). These methods, however, are neither completely specific nor accurate, and many other protease inhibitors in plasma inhibit tPA (4). Although several assays have also been described for PAI-1 antigen that are based on two-step enzyme immunoassays using monoclonal and/or polyclonal antibodies (5)(6), they have a narrow measurement range (0–40 μg/L) and are time-consuming (7). Because PAI-1 is a labile molecule, the utmost care is needed during blood collection and sample handling to ensure accurate measurement of PAI-1 and tPA. The half-life for the transformation from the active form of PAI-1 into the inactive latent form is ∼4 h in vitro and even shorter in vivo (8). It is therefore essential to correctly evaluate the amount of PAI-1 released from endothelial cells and adipose tissue (9) to make such methods clinically useful. We have developed a latex photometric immunoassay (LPIA) for total PAI-1 within a dynamic measurement range that can detect all forms of PAI-1 without the influence of conformational changes.
Samples were obtained from 47 patients with myocardial infarction (29 men and 18 women) and 276 hospital employees, who volunteered for this study, as controls [100 men (age range, 23–63 years) and 168 women (age range, 21–57 years)]. After informed consent, blood samples were collected in a 1/10 volume of a solution containing 38 g/L sodium …
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影响因子：
19.6
作者：
WitkoSarsat, V;Friedlander, M;DescampsLatscha, B
通讯作者：
DescampsLatscha, B
影响因子：
8.7
作者：
Voutilainen, S;Morrow, JD;Salonen, JT
通讯作者：
Salonen, JT
影响因子：
9.8
作者：
Powers, RW;Majors, AK;Roberts, JM
通讯作者：
Roberts, JM
影响因子：
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作者：
Zhu, YH;Carmeliet, P;Fay, WP
通讯作者：
Fay, WP