The Grape VlWRKY3 Gene Promotes Abiotic and Biotic Stress Tolerance in Transgenic Arabidopsis thaliana.
The Grape VlWRKY3 Gene Promotes Abiotic and Biotic Stress Tolerance in Transgenic Arabidopsis thaliana.
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葡萄 VlWRKY3 基因促进转基因拟南芥的非生物和生物胁迫耐受性
DOI：
10.3389/fpls.2018.00545
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Guo R;Qiao H;Zhao J;Wang X;Tu M;Guo C;Wan R;Li Z;Wang X
众所周知，WRKY转录因子在植物对各种非生物和生物胁迫的反应中起着重要作用。据报道，葡萄WRKY基因WRKY3对盐和干旱胁迫以及茉莉酸甲酯和乙烯处理有响应。“Kyoho。在本研究中，来自‘Kyoho’葡萄品种的WRKY3在花椰菜花叶病毒35S启动子的控制下在拟南芥中组成性表达。35S::VlWRKY3转基因拟南芥在萌发、苗期和植株成熟期均表现出较强的耐盐性和抗旱性。为了进一步了解VlWRKY3的作用，我们对与非生物胁迫应答相关的各种生理性状进行了评估，结果发现非生物胁迫对转基因植株的伤害小于野生型植株。VlWRKY3过表达也导致非生物应激反应基因的表达水平改变。此外，35S::VlWRKY3转基因拟南芥株系与野生型植株相比，对环horacearum葡萄球菌（Golovinomyces cichoracearum）的抗性增强，但对灰霉病（Botrytis cinerea）的敏感性增加。综上所述，这些结果表明，VlWRKY3在应对非生物和生物胁迫中都发挥着重要作用，修改其表达可能是提高作物抗逆性的一种策略。
WRKY transcription factors are known to play important roles in plant responses to various abiotic and biotic stresses. The grape WRKY gene, WRKY3 was previously reported to respond to salt and drought stress, as well as methyl jasmonate and ethylene treatments in Vitis labrusca × V. vinifera cv. ‘Kyoho.’ In the current study, WRKY3 from the ‘Kyoho’ grape cultivar was constitutively expressed in Arabidopsis thaliana under control of the cauliflower mosaic virus 35S promoter. The 35S::VlWRKY3 transgenic A. thaliana plants showed improved salt and drought stress tolerance during the germination, seedling and the mature plant stages. Various physiological traits related to abiotic stress responses were evaluated to gain further insight into the role of VlWRKY3, and it was found that abiotic stress caused less damage to the transgenic seedlings than to the wild-type (WT) plants. VlWRKY3 over-expression also resulted in altered expression levels of abiotic stress-responsive genes. Moreover, the 35S::VlWRKY3 transgenic A. thaliana lines showed improved resistance to Golovinomyces cichoracearum, but increased susceptibility to Botrytis cinerea, compared with the WT plants. Collectively, these results indicate that VlWRKY3 plays important roles in responses to both abiotic and biotic stress, and modification of its expression may represent a strategy to enhance stress tolerance in crops.
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影响因子：
7.3
作者：
Kotchoni, Simeon O.;Kuhns, Christine;Bartels, Dorothea
通讯作者：
Bartels, Dorothea
影响因子：
7.2
作者：
Bhattarai, Kishor K.;Atamian, Hagop S.;Eulgem, Thomas
通讯作者：
Eulgem, Thomas
影响因子：
11.6
作者：
Chen, Yi-Fang;Li, Li-Qin;Wu, Wei-Hua
通讯作者：
Wu, Wei-Hua
影响因子：
4.3
作者：
Lagacé, M;Matton, DP
通讯作者：
Matton, DP
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF