Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius
Generation of immunoglobulin heavy chain diversity subsequent to cell surface immunoglobulin expression in the avian bursa of Fabricius
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禽法氏囊中细胞表面免疫球蛋白表达后免疫球蛋白重链多样性的产生
DOI：
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发表时间：
1989
影响因子：
15.3
通讯作者：
M. J. H. Ratcliffe
中科院分区：
文献类型：
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作者：
M. J. H. Ratcliffe
法氏法氏囊是产生抗体多样性的场所。将新生法氏囊细胞转移到经环磷酰胺处理的新生鸡体内，可使受体法氏囊与供体来源的法氏囊干细胞重建。这些干细胞表达细胞表面的IgM，在限制供者法氏囊干细胞数量的条件下，每个重建的法氏囊毛囊由单个前体细胞定植。在这些克隆卵泡中，Ig VH独特型CVH-1的表达是异质性的。在法氏囊内产生的多样性随后在外周B细胞室内被发现。因此，功能Ig H链多样性的产生是在Ig H链重排和表达之后发生的。
The avian bursa of Fabricius represents a site for the generation of antibody diversity. Transfer of neonatal bursal cells into cyclophosphamide-treated neonatal chickens results in reconstitution of recipient bursae with donor-derived bursal stem cells. These stem cells express cell surface IgM and, under conditions of limiting donor bursal stem cell numbers, each reconstituted bursal follicle is colonized by a single precursor cell. The expression of an Ig VH idiotype, CVH-1, was found to be heterogenous within such clonal follicles. The diversity generated within the bursa is subsequently found within the peripheral B cell compartment. Thus, the generation of functional Ig H chain diversity is shown to occur subsequent to Ig H chain rearrangement and expression.
使用单克隆抗小鼠免疫球蛋白检测小鼠抗体。
影响因子：
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作者：
Yelton,DE;Desaymard,C;Scharff,MD
通讯作者：
Scharff,MD