The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis.
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis.
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DOI：
10.1186/1476-9255-11-11
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
da Cunha S
中科院分区：
文献类型：
--
作者：
de Andrade JAA;Gayer CRM;Nogueira NPA;Paes MC;Bastos VLFC;Neto JDCB;Alves SC Jr;Coelho RM;da Cunha MGAT;Gomes RN;Águila MB;Mandarim-de-Lacerda CA;Bozza PT;da Cunha S
脓毒症是危重患者的常见疾病，可能与硫胺素缺乏症（TD）有关。本研究的目的是评估TD对脓毒症模型中的炎症、氧化应激和细胞募集的影响。实验脓毒症模型，盲肠结扎和穿孔（CLP），用于小鼠与假手术相比。将以下四个组相互比较：用AIN93G完全食物的SHAM、用硫胺素缺乏（TD）食物的SHAM、用AIN93G完全食物的CLP和用TD食物的CLP。测定焦磷酸硫胺素（TPP）的血液浓度，并评价血液和腹膜液中TNF-α、IL-1、IL-6、KC和MCP-1/CCL2水平的差异。此外，通过蛋白质印迹法评价肝蛋白中4-HNE加合物的水平。喂食全价饲料的小鼠的平均TPP血液浓度为303.3 ± 42.6 nmol/L，TD发生在10天内。CLP + TD组腹腔液中TNF-α和MCP-1浓度显著高于其他组。而CLP + TD组血IL-1 β水平较低。肝脏4-HNE水平在TD食物组中最高。血液单核细胞数量以及腹膜总白细胞、单核细胞和中性粒细胞数量在CLP + TD饲料组中更大。腹腔细菌菌落形成单位（CFU）显着较低的CLP与TD饲料组。TD与更大的细菌清除、氧化应激和炎症反应变化相关。
Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model. The experimental sepsis model, cecal ligation and puncture (CLP), was utilized on mice in comparison with a sham procedure. The following four groups were compared against each other: SHAM with AIN93G complete chow, SHAM with thiamine deficient (TD) chow, CLP with AIN93G complete chow, and CLP with TD chow. Thiamine pyrophosphate (TPP) blood concentrations were determined, and blood and peritoneal fluid were evaluated for differences in TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 levels. In addition, the levels of 4-HNE adducts in liver proteins were evaluated by Western Blot. The mean TPP blood concentration from the mice fed with the complete chow was 303.3 ± 42.6 nmol/L, and TD occurred within 10 days. TNF-α and MCP-1 concentrations in the peritoneal fluid were significantly greater in the CLP with TD chow group when compared with the other groups. The blood IL-1β level, however, was lower in the CLP with TD chow group. Liver 4-HNE levels were highest in the TD chow groups. Blood mononuclear cell numbers, as well as peritoneal total leukocyte, mononuclear cell and neutrophil numbers were greater in the CLP with TD chow group. Peritoneal bacterial colony forming units (CFU) were significantly lower in the CLP with TD chow group. TD was associated with greater bacterial clearance, oxidative stress and inflammatory response changes.
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影响因子：
8.8
作者：
LINDENBAUM, GA;LARRIEU, AJ;KAPUSNICK, RA
通讯作者：
KAPUSNICK, RA
影响因子：
4.2
作者：
Karuppagounder, Saravanan S.;Xu, Hui;Gibson, Guy E.
通讯作者：
Gibson, Guy E.
DOI：
10.1186/cc5783
发表时间：
2007
期刊：
Critical care (London, England)
影响因子：
--
作者：
Bozza FA;Salluh JI;Japiassu AM;Soares M;Assis EF;Gomes RN;Bozza MT;Castro-Faria-Neto HC;Bozza PT
通讯作者：
Bozza PT
影响因子：
38.9
作者：
Engel, Christoph;Brunkhorst, Frank M.;Reinhart, Konrad
通讯作者：
Reinhart, Konrad
影响因子：
8.8
作者：
Angus, D C;Linde-Zwirble, W T;Pinsky, M R
通讯作者：
Pinsky, M R