Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein
Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein
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在潜伏生长转化病毒感染中转录的 mRNA 的核苷酸序列表明它可能编码膜蛋白
作者：
S. Fennewald;V. Santen;E. Kieff
在潜伏感染的细胞系IB 4中，最丰富的EB病毒mRNA是由基因组右端附近的主要独特DNA编码的2，8-三磷酸腺苷酶RNA，IB 4是通过病毒体外生长转化建立的。RNA从右向左转录，两个内含子被剪接掉。对该基因组区域进行测序，并通过DNA-RNA杂交的S1分析和引物延伸鉴定RNA的外显子。RNA中的第一个起始密码子是从其5'端开始的40个核苷酸。从起始密码子开始，有一个1，158个核苷酸的开放阅读框架，它穿过两个内含子。翻译蛋白的重要特征如下。(i)氨基末端高度带电，不提示前导序列。(ii)有6个明显疏水的α-螺旋结构域，每个结构域具有21个氨基酸，并由5至7个氨基酸片段连接，预测为反向转角。(iii)羧基端200个氨基酸显着酸性，含有6个谷氨酸和37个天冬氨酸。预测疏水区域形成六个跨膜区域，在膜内侧留下短的带电氨基末端和长的酸性羧基末端。该蛋白可能是EB病毒转化细胞质膜中检测到的新抗原，即淋巴细胞决定的膜抗原。RNA中还有另外两个开放阅读框。
The most abundant Epstein-Barr virus mRNA in a latently infected cell line, IB4, established by in vitro growth transformation with virus, was a 2,8-kilobase RNA encoded by largely unique DNA near the right end of the genome. The RNA was transcribed from right to left, and two introns were spliced out. This region of the genome was sequenced, and the exons of the RNA were identified by S1 analysis of DNA-RNA hybrids and primer extension. The first start codon in the RNA was 40 nucleotides from its 5' end. Beginning with the start codon, there was a 1,158-nucleotide open reading frame which crossed both introns. The important characteristics of the translated protein were as follows. (i) The amino terminus was highly charged and not suggestive of a leader sequence. (ii) There were six markedly hydrophobic alpha-helical domains, each having 21 amino acids and connected by 5 to 7 amino acid segments predicted to be reverse turns. (iii) The carboxy-terminal 200 amino acids were markedly acidic, containing 6 glutamic and 37 aspartic acids. The hydrophobic region is predicted to form six membrane-spanning regions, leaving the short charged amino terminus and long acidic carboxy terminus on the inside of the membrane. This protein could be responsible for the new antigen detected in the plasma membrane of Epstein-Barr virus-transformed cells, lymphocyte-determined membrane antigen. There were two other open reading frames in the RNA.
DOI：
10.1073/pnas.80.7.1987
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
HENDERSON, A;RIPLEY, S;KIEFF, E
通讯作者：
KIEFF, E