Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat
Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat
复制标题
人类多能间充质干细胞可改善大鼠胶原酶肌腱损伤后的愈合
DOI：
--
复制
发表时间：
2014
影响因子：
3.9
通讯作者：
P. Jendelová
中科院分区：
文献类型：
--
作者：
Lucia Machová Urdzíková;R. Sedláček;T. Suchý;T. Amemori;J. Ruzicka;P. Lesný;V. Havlas;E. Sykova;P. Jendelová
间充质基质细胞由于其具有向中胚层分化的能力、旁分泌特性以及在自体移植中的应用前景，在组织再生领域引起了广泛的关注。本研究的目的是探讨植入的人间充质基质细胞（hMSCs）的安全性和修复潜力，良好的生产规范（GMP）的条件下，利用人混合血小板裂解液作为培养补充剂，在胶原酶跟腱损伤模型rats.MethodsEighty-one胶原酶诱导的损伤大鼠分为两组。第一组在损伤诱导后3天接受人间充质基质细胞注射到损伤部位，而第二组接受生理盐水。生物力学测试，形态测定和半定量免疫组化胶原蛋白I，II和III，多能蛋白聚糖和聚集蛋白聚糖，新生血管，和hMSC生存进行2，4，6 weeks after injury.ResultsHuman mesenchymal stromal cells-treated rats had a significant better extracellular matrix structure and a large amount of collagen I and collagen III.在肌腱损伤后2周和4周，hMSC治疗的大鼠中新生血管也增加。MTCO 2（细胞色素c氧化酶亚基II）阳性证实了移植后2、4和6周hMSC的存在。胶原蛋白II沉积和茜素红染色的骨被发现在6 hMSC和2盐水处理的肌腱损伤后6周。抗多功能蛋白聚糖和抗聚集蛋白聚糖染色的强度没有不同groups.ConclusionshMSCs可以支持肌腱愈合，通过更好的血管化，以及通过更大的存款和更好的组织的细胞外基质。发现治疗程序是安全的;但是，在计划随后的肌腱病体内和临床试验时，应将植入部位的软骨和骨形成作为预期不良事件考虑在内。
BackgroundMesenchymal stromal cells attract much interest in tissue regeneration because of their capacity to differentiate into mesodermal origin cells, their paracrine properties and their possible use in autologous transplantations. The aim of this study was to investigate the safety and reparative potential of implanted human mesenchymal stromal cells (hMSCs), prepared under Good Manufacturing Practice (GMP) conditions utilizing human mixed platelet lysate as a culture supplement, in a collagenase Achilles tendon injury model in rats.MethodsEighty-one rats with collagenase-induced injury were divided into two groups. The first group received human mesenchymal stromal cells injected into the site of injury 3 days after lesion induction, while the second group received saline. Biomechanical testing, morphometry and semiquantitative immunohistochemistry of collagens I, II and III, versican and aggrecan, neovascularization, and hMSC survival were performed 2, 4, and 6 weeks after injury.ResultsHuman mesenchymal stromal cell-treated rats had a significantly better extracellular matrix structure and a larger amount of collagen I and collagen III. Neovascularization was also increased in hMSC-treated rats 2 and 4 weeks after tendon injury. MTCO2 (Cytochrome c oxidase subunit II) positivity confirmed the presence of hMSCs 2, 4 and 6 weeks after transplantation. Collagen II deposits and alizarin red staining for bone were found in 6 hMSC- and 2 saline-treated tendons 6 weeks after injury. The intensity of anti-versican and anti-aggrecan staining did not differ between the groups.ConclusionshMSCs can support tendon healing through better vascularization as well as through larger deposits and better organization of the extracellular matrix. The treatment procedure was found to be safe; however, cartilage and bone formation at the implantation site should be taken into account when planning subsequent in vivo and clinical trials on tendinopathy as an expected adverse event.
影响因子：
4.2
作者：
BASSO, DM;BEATTIE, MS;BRESNAHAN, JC
通讯作者：
BRESNAHAN, JC