Analysis of tissue and serum microRNA expression in patients with upper urinary tract urothelial cancer.
Analysis of tissue and serum microRNA expression in patients with upper urinary tract urothelial cancer.
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DOI：
10.1371/journal.pone.0117284
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ellinger J
中科院分区：
文献类型：
--
作者：
Kriebel S;Schmidt D;Holdenrieder S;Goltz D;Kristiansen G;Moritz R;Fisang C;Müller SC;Ellinger J
microRNA在许多人类恶性肿瘤中起重要作用;到目前为止，其在上尿路尿路上皮癌（UUTUC）中的表达仍有待研究。在接受UUTUC肾输尿管切除术的患者的相应正常和癌组织样本中研究了先前显示在尿路上皮膀胱癌中上调的11种microRNA（miR-10a、miR-21、miR-96、miR-135、miR-141、miR-182、miR-200 b、miR-205、miR-429、miR-520 b、miR-1244）的表达。然后在UUTUC患者和非恶性泌尿系统疾病患者的血清样品中测量上调的microRNA，以评估其作为UUTUC非侵入性生物标志物的潜力。microRNA表达允许正常和癌组织的分化：miR-21、miR-96、miR-135、miR-141、miR-182、miR-205、miR-429和miR-520 b显著过表达。此外，miR-205在低分化UUTUC中上调。血清中循环RNA的分析表明UUTUC患者中miR-141增加;受试者操作者特征分析表明miR-141作为诊断生物标志物的曲线下面积为0.726。此外，我们观察到UUTUC患者的miR-10a和miR-135水平较低。UUTUC中microRNA表达改变。循环miR-141的分析可能有助于识别UUTUC患者。
MicroRNAs play an important role in many human malignancies; so far, their expression remains to be studied in upper urinary tract urothelial cancer (UUTUC). The expression of eleven microRNAs (miR-10a, miR-21, miR-96, miR-135, miR-141, miR-182, miR-200b, miR-205, miR-429, miR-520b, miR-1244) formerly shown to be upregulated in urothelial bladder cancer were studied in corresponding normal and cancerous tissue samples of patients undergoing nephroureterectomy for UUTUC. Upregulated microRNAs were then measured in serum samples of patients with UUTUC and patients with non-malignant urological diseases to evaluate their potential as non-invasive biomarkers for UUTUC. MicroRNA expression allowed differentiation of normal and cancerous tissue: miR-21, miR-96, miR-135, miR-141, miR-182, miR-205, miR-429 and miR-520b were significantly overexpressed. Furthermore, miR-205 was upregulated in poorly differentiated UUTUC. The analysis of circulating RNA in serum demonstrated an increase of miR-141 in patients with UUTUC; receiver operator characteristic analysis demonstrated an area under the curve of 0.726 for miR-141 as a diagnostic biomarker. Furthermore, we observed lower levels of miR-10a and miR-135 in UUTUC patients. MicroRNA expression is altered in UUTUC. The analysis of circulating miR-141 may be useful to identify patients with UUTUC.
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