Nucleus Reuniens Afferents in Hippocampus Modulate CA1 Network Function via Monosynaptic Excitation and Polysynaptic Inhibition.
Nucleus Reuniens Afferents in Hippocampus Modulate CA1 Network Function via Monosynaptic Excitation and Polysynaptic Inhibition.
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DOI：
10.3389/fncel.2021.660897
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发表时间：
2021
影响因子：
5.3
通讯作者：
McQuiston AR
中科院分区：
文献类型：
--
作者：
Goswamee P;Leggett E;McQuiston AR
丘脑中线核通过向腔隙分子层（SLM）的密集投射调节海马CA 1区和下托的功能。以前，解剖数据表明，在SLM reuniens输入形式突触与树突的CA 1主细胞和抑制性中间神经元。然而，丘脑输入的能力，兴奋CA 1主细胞仍然存在争议。此外，还不知道reuniens输入对CA 1中间神经元不同亚群的影响。因此，在野生型和转基因小鼠的离体海马切片中使用全细胞膜片钳电生理学，我们测量了不同CA 1神经元亚型对光遗传学刺激reuniens传入的突触反应。我们的数据表明，reuniens输入介导的兴奋和抑制的CA 1主细胞。然而，团聚体输入的光遗传学激发未能驱动大多数主细胞中的动作电位放电。虽然兴奋性突触后电流介导的直接单突触激活的CA 1主细胞，抑制性突触后电流产生多突触通过激活本地GABA能中间神经元。此外，我们证明，光遗传学刺激reuniens输入差异招募至少两个不同的和非重叠的亚群的本地GABA能中间神经元在CA 1。我们发现，位于SLM的神经胶质细胞，和calretinin在SLM/放射层边界的选择性中间神经元的神经胶质细胞可以通过刺激reuniens输入兴奋。总之，我们的数据表明，reuniens传入的光遗传学刺激可以介导兴奋，前馈抑制，并通过多种直接和间接机制的突触后CA 1主细胞的去抑制。
The thalamic midline nucleus reuniens modulates hippocampal CA1 and subiculum function via dense projections to the stratum lacunosum-moleculare (SLM). Previously, anatomical data has shown that reuniens inputs in the SLM form synapses with dendrites of both CA1 principal cells and inhibitory interneurons. However, the ability of thalamic inputs to excite the CA1 principal cells remains controversial. In addition, nothing is known about the impact of reuniens inputs on diverse subpopulations of interneurons in CA1. Therefore, using whole cell patch-clamp electrophysiology in ex vivo hippocampal slices of wild-type and transgenic mice, we measured synaptic responses in different CA1 neuronal subtypes to optogenetic stimulation of reuniens afferents. Our data shows that reuniens inputs mediate both excitation and inhibition of the CA1 principal cells. However, the optogenetic excitation of the reuniens inputs failed to drive action potential firing in the majority of the principal cells. While the excitatory postsynaptic currents were mediated via direct monosynaptic activation of the CA1 principal cells, the inhibitory postsynaptic currents were generated polysynaptically via activation of local GABAergic interneurons. Moreover, we demonstrate that optogenetic stimulation of reuniens inputs differentially recruit at least two distinct and non-overlapping subpopulations of local GABAergic interneurons in CA1. We show that neurogliaform cells located in SLM, and calretinin-containing interneuron-selective interneurons at the SLM/stratum radiatum border can be excited by stimulation of reuniens inputs. Together, our data demonstrate that optogenetic stimulation of reuniens afferents can mediate excitation, feedforward inhibition, and disinhibition of the postsynaptic CA1 principal cells via multiple direct and indirect mechanisms.
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影响因子：
4.7
作者：
Bell, Karen A.;Shim, Hoon;Chen, Ching-Kang;McQuiston, A. Rory
通讯作者：
McQuiston, A. Rory
影响因子：
3.4
作者：
Lin, John Y.;Lin, Michael Z.;Tsien, Roger Y.
通讯作者：
Tsien, Roger Y.
影响因子：
25
作者：
Li, Yiding;Xu, Jiamin;Zhang, Xiaohui
通讯作者：
Zhang, Xiaohui
影响因子：
7.7
作者：
Jankowski MM;Islam MN;Wright NF;Vann SD;Erichsen JT;Aggleton JP;O'Mara SM
通讯作者：
O'Mara SM
影响因子：
5.3
作者：
Price, CJ;Cauli, B;Capogna, M
通讯作者：
Capogna, M