Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice.
Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice.
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DOI：
10.1007/s10439-010-0114-3
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发表时间：
2010-12
影响因子：
3.8
通讯作者：
Gleason, Rudolph L., Jr.
中科院分区：
文献类型：
--
作者：
Wan, William;Yanagisawa, Hiromi;Gleason, Rudolph L., Jr.
动脉力学特性的改变伴随着衰老和疾病发生，动脉硬化是随后心血管事件的关键危险因素。动脉硬化与功能性弹性纤维的丧失和大动脉壁胶原蛋白含量的增加有关。动脉的机械性能主要由关键结构蛋白和细胞的周转和重组控制，这一过程被称为生长和重塑。纤维蛋白-5 （fbln5）是一种微纤维蛋白，可结合弹力蛋白，与整合素相互作用，并定位于弹力蛋白纤维；弹力蛋白和微纤维蛋白构成功能性弹性纤维。我们对纤维蛋白-5缺失小鼠（fbln5−/−）和同窝对照小鼠（fbln5+/+）的颈总动脉（CCAs）进行了双轴力学测试和共聚焦成像，以表征这些动脉的力学行为和微观结构含量；力学试验数据符合四纤维族本构模型。我们发现来自fbln5 - / -小鼠的CCAs表现出较低的体内轴向拉伸和较低的体内应激，同时与同窝对照相比，对生理压力保持相似的依从性。其中，fbln5−/−的轴向拉伸λ = 1.41±0.07，周向应力σθ = 101±32 kPa，轴向应力σz = 74±28 kPa；为fbln5+/+ λ = 1.64±0.03,σθ = 194±38 kPa, σz = 159±29 kPa。在结构上，来自fbln5 - / -小鼠的CCAs缺乏明显的功能性弹性纤维，由平滑肌细胞和弹性蛋白片交替层的层状结构定义。这些数据表明fbln5 - / -动脉的结构差异与力学性能的显著差异相关。尽管存在这些显著差异，fbln5−/−cca在心脏周期内表现出接近正常水平的循环应变。
Alteration in the mechanical properties of arteries occurs with aging and disease, and arterial stiffening is a key risk factor for subsequent cardiovascular events. Arterial stiffening is associated with the loss of functional elastic fibers and increased collagen content in the wall of large arteries. Arterial mechanical properties are controlled largely by the turnover and reorganization of key structural proteins and cells, a process termed growth and remodeling. Fibulin-5 (fbln5) is a microfibrillar protein that binds tropoelastin, interacts with integrins, and localizes to elastin fibers; tropoelastin and microfibrillar proteins constitute functional elastic fibers. We performed biaxial mechanical testing and confocal imaging of common carotid arteries (CCAs) from fibulin-5 null mice (fbln5−/−) and littermate controls (fbln5+/+) to characterize the mechanical behavior and microstructural content of these arteries; mechanical testing data were fit to a four-fiber family constitutive model. We found that CCAs from fbln5−/− mice exhibited lower in vivo axial stretch and lower in vivo stresses while maintaining a similar compliance over physiological pressures compared to littermate controls. Specifically, for fbln5−/− the axial stretch λ = 1.41 ± 0.07, the circumferential stress σθ = 101 ± 32 kPa, and the axial stress σz = 74 ± 28 kPa; for fbln5+/+ λ = 1.64 ± 0.03, σθ = 194 ± 38 kPa, and σz = 159 ± 29 kPa. Structurally, CCAs from fbln5−/− mice lack distinct functional elastic fibers defined by the lamellar structure of alternating layers of smooth muscle cells and elastin sheets. These data suggest that structural differences in fbln5−/− arteries correlate with significant differences in mechanical properties. Despite these significant differences fbln5−/− CCAs exhibited nearly normal levels of cyclic strain over the cardiac cycle.
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影响因子：
2
作者：
Holzapfel, GA;Gasser, TC;Ogden, RW
通讯作者：
Ogden, RW
影响因子：
2.4
作者：
DOBRIN, PB
通讯作者：
DOBRIN, PB
影响因子：
4.3
作者：
Fillinger, MF;Racusin, J;Rzucidlo, EM
通讯作者：
Rzucidlo, EM
DOI：
10.1152/ajpheart.00046.2005
发表时间：
2005-09-01
影响因子：
4.8
作者：
Wagenseil, JE;Nerurkar, NL;Mecham, RP
通讯作者：
Mecham, RP
影响因子：
64.8
作者：
Nakamura, T;Lozano, PR;Chien, KR
通讯作者：
Chien, KR