Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.
Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.
复制标题
肠道细胞外基质相关信号在猪流行性腹泻病毒感染中的作用。
DOI：
10.1080/21505594.2021.1972202
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
Li Y;Li J;Wang X;Wu Q;Yang Q
Porcine epidemic diarrhea virus (PEDV) is emerging as a major threat to the global swine industry. Clinical PEDV infection is associated with severe intestinal lesions, resulting in absorptive dysfunction and high mortality rates in suckling piglets. The extracellular matrix (ECM) is an important component of intestinal tissue, providing a structural framework and conveying tissue-specific signals to nearby enterocytes. In this study, we investigated the extensive ECM remodeling observed in intestinal epithelial cells infected with PEDV and elucidated the associated activated ECM receptor-related pathways. Protein-protein interaction network analysis revealed two significantly differentially expressed genes (cluster of differentiation 44 [CD44] and serpin family E member 1 [SERPINE1]) associated with the ECM. At the transcriptional level, both genes exhibited significant positive correlation with the extent of PEDV replication. Similarly, the expression of CD44 and PAI-1 (encoded by SERPINE1) was also increased in the intestines of piglets during viral infection. Furthermore, CD44 exhibited antiviral activity by enhancing the expression of antiviral cytokines (e.g., interleukin [IL]-6, IL-18, IL-11, and antimicrobial peptide beta-defensin 1) by activating nuclear factor-κB signaling. Conversely, PAI-1 was found to promote the release of progeny virions during PEDV infection, despite a decreased intracellular viral load. Nevertheless, the underlying mechanisms are still unclear. Taken together, our results highlighted the biological roles of specific ECM-regulated genes, i.e., CD44 and SERPINE1 in suppressing and promoting PEDV infection, thereby providing a theoretical foundation for the role of the ECM in intestinal infections and identifying potential therapeutic targets for PEDV.
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影响因子：
3.7
作者：
Ding Z;An K;Xie L;Wu W;Zhang R;Wang D;Fang Y;Chen H;Xiao S;Fang L
通讯作者：
Fang L
影响因子：
5.6
作者：
Ghosh, Asish K.;Vaughan, Douglas E.
通讯作者：
Vaughan, Douglas E.
DOI：
10.1073/pnas.1307336110
发表时间：
2013-11-05
影响因子：
11.1
作者：
Fouda, Genevieve G.;Jaeger, Frederick H.;Permar, Sallie R.
通讯作者：
Permar, Sallie R.
DOI：
10.1016/j.biocel.2016.09.009
发表时间：
2016-12-01
影响因子：
4
作者：
Morath, I.;Hartmann, T. N.;Orian-Rousseau, V.
通讯作者：
Orian-Rousseau, V.
DOI：
10.1186/1476-9255-10-23
发表时间：
2013-05-30
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Black KE;Collins SL;Hagan RS;Hamblin MJ;Chan-Li Y;Hallowell RW;Powell JD;Horton MR
通讯作者：
Horton MR