Combined bulked segregant sequencing and traditional linkage analysis for identification of candidate gene for purple leaf sheath in maize.
Combined bulked segregant sequencing and traditional linkage analysis for identification of candidate gene for purple leaf sheath in maize.
复制标题
联合批量分离测序和传统连锁分析鉴定玉米紫色叶鞘候选基因
DOI:
10.1371/journal.pone.0190670
复制
发表时间:
2018
期刊:
影响因子:
3.7
通讯作者:
Yang Z
中科院分区:
文献类型:
--
作者:
Li P;Du C;Zhang Y;Yin S;Zhang E;Fang H;Lin D;Xu C;Yang Z
Anthocyanin accumulation in various maize tissues plays important roles in plant growth and development. In addition, some color-related traits can be used as morphological markers in conventional maize breeding processes and purity identification of hybrid seeds. Here, we noticed that the leaf sheath color was controlled by a dominant gene, because purple (PSH) and green leaf sheaths (GSH) were separated at a ratio of 3:1 in an F2 population. To map the gene, an F2 and a recombinant inbred line (RIL) population were derived from a cross between inbred line T877 (PSH) and DH1M (GSH). The PSH locus was mapped to the genomic region within 128.8 to 138.4 Mb using a bulked segregant sequencing approach. This position was further validated by linkage mapping using 190 F2 plants with GSH. Subsequently, the PSH locus was fine-mapped into an interval of 304.2 kb. A maize gene, GRMZM5G822829, was identified in this region, encoding a bHLH transcription factor. The expression level of this gene in T877 was found to be 9-fold higher than that of DH1M. In conclusion, our results suggest that GRMZM5G822829 is the putative candidate gene conferring leaf sheath color in maize.
登录
查看更多内容
影响因子:
4.4
作者:
Liu H;Qin C;Chen Z;Zuo T;Yang X;Zhou H;Xu M;Cao S;Shen Y;Lin H;He X;Zhang Y;Li L;Ding H;Lübberstedt T;Zhang Z;Pan G
通讯作者:
Pan G
DOI:
10.1111/j.1365-313x.2006.02964.x
发表时间:
2007-02
期刊:
The Plant journal : for cell and molecular biology
影响因子:
--
作者:
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者:
Allan AC
影响因子:
14.9
作者:
GIOVANNONI, JJ;WING, RA;TANKSLEY, SD
通讯作者:
TANKSLEY, SD
影响因子:
7
作者:
Huang, Xuehui;Feng, Qi;Han, Bin
通讯作者:
Han, Bin
DOI:
10.1073/pnas.90.17.8244
发表时间:
1993-09-01
影响因子:
11.1
作者:
DAMIANI, RD;WESSLER, SR
通讯作者:
WESSLER, SR