Development of an antioxidant assay to study oxidative potential of airborne particulate matter
Development of an antioxidant assay to study oxidative potential of airborne particulate matter
复制标题
开发抗氧化剂测定法来研究空气颗粒物的氧化潜力
DOI：
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jake Wilson
中科院分区：
文献类型：
--
作者：
Pourya Shahpoury;T. Harner;G. Lammel;Steven Lelieveld;Haijie Tong;Jake Wilson
摘要。氧化电位是空气中氧化还原活性的量度
Abstract. Oxidative potential is a measure of redox activity of airborne
particulate matter (PM) and is often used as a surrogate to estimate one
form of PM toxicity. The evaluation of oxidative potential in
a physiologically relevant environment is always challenging. In this work, we
developed a chromatographic method, employing an ultra-high-performance
liquid chromatograph coupled to a triple–quadruple mass spectrometer, to
determine the oxidative potential of PM from different sources. To this
purpose, we measured the PM-induced oxidation of glutathione, cysteine, and
ascorbic acid, and formation of glutathione disulfide and cystine, following
PM addition to simulated epithelial lining fluids, which, in addition to the
antioxidants, contained inorganic salts, a phospholipid, and proteins. The
new method showed high precision and, when applied to standard reference PM,
the oxidative potential was found to increase with the reaction time and PM
concentration in the lung fluid. The antioxidant depletion rates were
considerably higher than the rates found with the conventional
dithiothreitol assay, indicating the higher sensitivity of the new method.
The presence of the lung fluid inorganic species increased the oxidative
potential determined through glutathione and cysteine, but showed an
opposite effect with ascorbic acid, whereas the presence of proteins
resulted in a moderate decrease in the oxidative potential. In the presence
of PM 2.5 , glutathione and cysteine demonstrated similar depletion
patterns, which were noticeably different from that of ascorbic acid,
suggesting that cysteine could be used as an alternative to glutathione for
probing oxidative potential.
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影响因子：
7.4
作者：
Song, Yang;Buettner, Garry R.
通讯作者：
Buettner, Garry R.
DOI：
10.1165/rcmb.2007-0447oc
发表时间：
2009-01-01
影响因子：
6.4
作者：
Iyer, Smita S.;Jones, Dean P.;Rojas, Mauricio
通讯作者：
Rojas, Mauricio
DOI：
10.1016/j.jchromb.2016.02.015
发表时间：
2016-04-15
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
Giustarini D;Tsikas D;Colombo G;Milzani A;Dalle-Donne I;Fanti P;Rossi R
通讯作者：
Rossi R
影响因子：
3.4
作者：
Haijie Tong;P. Lakey;Andrea M. Arangio;Joanna Socorro;C. Kampf;T. Berkemeier;W. Brune;U. Pöschl;M. Shiraiwa
通讯作者：
Haijie Tong;P. Lakey;Andrea M. Arangio;Joanna Socorro;C. Kampf;T. Berkemeier;W. Brune;U. Pöschl;M. Shiraiwa
影响因子：
10.4
作者：
Cross CE;van der Vliet A;O'Neill CA;Louie S;Halliwell B
通讯作者：
Halliwell B