Preparation and functional evaluation of monoclonal antibodies targeting Hepatitis B Virus Polymerase.
Preparation and functional evaluation of monoclonal antibodies targeting Hepatitis B Virus Polymerase.
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DOI：
10.1080/21505594.2020.1869391
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Tian D
中科院分区：
文献类型：
--
作者：
Hu S;Xiong H;Kang X;Wang S;Zhang T;Yuan Q;Tian D
HBV pol在B型肝炎病毒（HBV）的复制中起着关键作用。由于缺乏有效的检测方法，以往对HBV pol的研究对HBV pol表达的证据有限。本研究以HBV pol（159-406 aa）蛋白为靶点，筛选识别HBV pol的特异性单克隆抗体，并评价其诊断和治疗价值。用ELISA法检测HBV pol蛋白，获得4株抗HBV pol的抗体（P3、P5、P12、P20）。其中，P20抗体显示与HBV pol的最佳结合，如在用HBV基因组转染的细胞模型中通过蛋白质印迹（WB）所证明的。我们还通过转染的方法在小鼠肝细胞中表达了P5和P12抗体，结果表明这两种抗体具有显著的抗病毒作用，尤其是P12。综上所述，本研究建立了一种抗体，命名为P20。该抗体可用于通过WB分析检测四种HBV基因组的HBV pol表达。另外，P12抗体可通过细胞内表达发挥抗病毒作用，为慢性B型肝炎的治疗提供了一条新的途径。
HBV pol plays a critical role in the replication of hepatitis B virus (HBV). Previous studies conducted on HBV pol have produced limited evidence on HBV pol expression due to the lack of effective detection methods. The present study used the HBV pol (159–406 aa) protein as a target to screen for specific monoclonal antibodies that recognize HBV pol and subsequently evaluate their diagnostic and therapeutic value. Four antibodies (P3, P5, P12, P20) against HBV pol were obtained. Among them, the P20 antibody indicated optimal binding with HBV pol as demonstrated by Western blotting (WB) in a cell model transfected with the HBV genome. We also expressed P5 and P12 antibodies in mouse liver cells by transfection and the results indicated significant antiviral effects caused by these two antibodies especially P12. In summary, the present study established an antibody which was denoted P20. This antibody can be used to detect HBV pol expression by four HBV genomes via WB analysis. In addition, the antibody denoted P12 could exert antiviral effects via intracellular expression, which may provide a promising approach for the treatment of chronic hepatitis B.
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