Contributions of chaperone/usher systems to cell binding, biofilm formation and Yersinia pestis virulence.
Contributions of chaperone/usher systems to cell binding, biofilm formation and Yersinia pestis virulence.
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伴侣/Usher系统对细胞结合，生物膜形成和耶尔森氏菌毒力的贡献。
DOI：
10.1099/mic.0.044826-0
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Krukonis ES
中科院分区：
文献类型：
--
作者：
Felek S;Jeong JJ;Runco LM;Murray S;Thanassi DG;Krukonis ES
鼠疫耶尔森氏菌基因组测序项目揭示了六个完整的、未表征的伴侣/引导系统，有可能在鼠疫发病机制中发挥作用。我们克隆了每个基因座，并在 Δfim 大肠杆菌菌株 AAEC185 中表达它们，以测试组装的鼠疫耶尔森氏菌表面结构的各种活性。每个伴侣/引座基因座的表达都会在大肠杆菌表面产生特定的新型纤维结构。一个位点 y0561-0563 能够介导与人上皮细胞 (HEp-2) 和人巨噬细胞 (THP-1) 的附着，但不能介导小鼠巨噬细胞 (RAW264.7) 的附着，而几个位点能够促进大肠杆菌生物膜的形成。当鼠疫耶尔森氏菌中的每个伴侣/引座基因座被删除时，仅删除先前描述的pH 6抗原（Psa）伴侣/引座系统会导致粘附和生物膜形成减少。定量 RT-PCR (qRT-PCR) 显示每种新型伴侣/引导系统在体外以及静脉感染后的小鼠组织中的表达水平较低。然而，陪伴/引座基因座 y1858-1862 中的鼠疫耶尔森氏菌突变体通过静脉内感染途径在小鼠中的毒力减弱，表明该基因座的表达在某个阶段足以影响鼠疫感染的结果。 qRT-PCR 实验还表明，伴侣/引座依赖型荚膜基因座 caf1 的表达受到氧气可用性的影响，并且即使在 28°C 而不是 37°C（之前认为是 Psa 表达所需的温度）的基本培养基中，良好描述的伴侣/引座依赖型菌毛 Psa 也会被强烈诱导。这些数据表明鼠疫耶尔森氏菌的新型伴侣/引导系统在发病机制和感染相关功能（例如细胞粘附和生物膜形成）中的几个潜在作用。
Yersinia pestis genome sequencing projects have revealed six intact uncharacterized chaperone/usher systems with the potential to play roles in plague pathogenesis. We cloned each locus and expressed them in the Δfim Escherichia coli strain AAEC185 to test the assembled Y. pestis surface structures for various activities. Expression of each chaperone/usher locus gave rise to specific novel fibrillar structures on the surface of E. coli. One locus, y0561-0563, was able to mediate attachment to human epithelial cells (HEp-2) and human macrophages (THP-1) but not mouse macrophages (RAW264.7), while several loci were able to facilitate E. coli biofilm formation. When each chaperone/usher locus was deleted in Y. pestis, only deletion of the previously described pH 6 antigen (Psa) chaperone/usher system resulted in decreased adhesion and biofilm formation. Quantitative RT-PCR (qRT-PCR) revealed low expression levels for each novel chaperone/usher system in vitro as well as in mouse tissues following intravenous infection. However, a Y. pestis mutant in the chaperone/usher locus y1858-1862 was attenuated for virulence in mice via the intravenous route of infection, suggesting that expression of this locus is, at some stage, sufficient to affect the outcome of a plague infection. qRT-PCR experiments also indicated that expression of the chaperone/usher-dependent capsule locus, caf1, was influenced by oxygen availability and that the well-described chaperone/usher-dependent pilus, Psa, was strongly induced in minimal medium even at 28 °C rather than 37 °C, a temperature previously believed to be required for Psa expression. These data indicate several potential roles for the novel chaperone/usher systems of Y. pestis in pathogenesis and infection-related functions such as cell adhesion and biofilm formation.
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影响因子：
3.1
作者：
Felek, Suleyman;Krukonis, Eric S.
通讯作者：
Krukonis, Eric S.
影响因子：
5.1
作者：
Bobrov, Alexander G.;Kirillina, Olga;Perry, Robert D.
通讯作者：
Perry, Robert D.
影响因子：
3.2
作者：
BEESLEY, ED;BRUBAKER, RR;SURGALLA, MJ
通讯作者：
SURGALLA, MJ
影响因子：
3.1
作者：
DeBord, Kristin L.;Anderson, Deborah M.;Schneewind, Olaf
通讯作者：
Schneewind, Olaf
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL