Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC.
Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC.
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实时多路复用PCR的多中心评估用于检测碳青霉酶基因OXA-48，VIM，IMP，NDM和KPC。
DOI：
10.1186/1471-2334-14-27
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发表时间：
2014-01-14
影响因子：
3.7
通讯作者：
Ossewaarde JM
中科院分区：
文献类型：
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作者：
van der Zee A;Roorda L;Bosman G;Fluit AC;Hermans M;Smits PH;van der Zanden AG;Te Witt R;Bruijnesteijn van Coppenraet LE;Cohen Stuart J;Ossewaarde JM
Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for detection of the most prevalent carbapenemase genes; blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC. A real-time multiplex PCR assay was evaluated using a collection of 86 Gram negative isolates, including 62 carbapenemase producers. Seven different laboratories carried out this method and used the assay for detection of the carbapenemase genes on a selection of 20 isolates. Both sensitivity and specificity of the multiplex PCR assay was 100%, as established by results on the strain collection and the inter-laboratory comparisons. In this study, we present a multiplex real-time PCR that is a robust, reliable and rapid method for the detection of the most prevalent carbapenemases blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC, and is suitable for screening of broth cultured rectal swabs and for identification of carbapenemase genes in cultures.
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影响因子：
4.9
作者：
Cuzon, Gaelle;Ouanich, Jocelyne;Nordmann, Patrice
通讯作者：
Nordmann, Patrice
影响因子：
9.4
作者：
Chen, Liang;Mediavilla, Jose R.;Kreiswirth, Barry N.
通讯作者：
Kreiswirth, Barry N.
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作者：
van Der Bij, Akke K.;Mol, Marco;Pitout, Johann D. D.
通讯作者：
Pitout, Johann D. D.
影响因子：
4.9
作者：
Naas, Thierry;Ergani, Ayla;Nordmann, Patrice
通讯作者：
Nordmann, Patrice
影响因子：
5.2
作者：
Tsakris, Athanassios;Poulou, Aggeliki;Sofianou, Danai
通讯作者：
Sofianou, Danai