Measurement of light gradients and spectral regime in plant-tissue with a fiber optic probe
Measurement of light gradients and spectral regime in plant-tissue with a fiber optic probe
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使用光纤探头测量植物组织中的光梯度和光谱范围
DOI：
10.1111/j.1399-3054.1984.tb06076.x
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发表时间：
1984
影响因子：
6.4
通讯作者：
L. Björn
中科院分区：
文献类型：
--
作者：
T. Vogelmann;L. Björn
描述了一种方法，其中可以使用光纤测量植物组织内的光梯度和光谱状况。通过修改单根光纤（直径200 μm）制成光纤探针，使其具有直径为20-70 μm的截短尖端的光收集端。探头是具有17-20°的半带接收角的定向传感器。当光纤探针由机动显微操作器驱动通过植物组织时进行光测量，并且进入光纤尖端的光被输送到分光辐射计。通过辐照多汁植物黄花厚壳的绿色叶组织，利用准直光并从不同方向插入探针，可以测量不同深度的光质量和数量。准直光被最初1.0 mm的叶组织完全散射，这也极大地衰减了除绿色和远红光之外的所有光。光散射对光的数量有很大的贡献，并具有明显的光谱结构。在照射表面的正下方，550 nm处的光量是入射光的1.2倍。在1.4 mm深度处，光梯度迅速下降至0.5倍入射光。相比之下，在750 nm处的光量在初始0.5 mm至2.9倍入射光期间增加，然后在叶的暗侧（4.5 mm）线性下降至0.5倍入射光。还讨论了光散射对光梯度的贡献的大小的影响。
A method is described in which light gradients and spectral regime can be measured within plant tissue using fiber optics. A fiber optic probe was made by modifying a single optical fiber (200 μm diameter) so that it had a light harvesting end that was a truncated tip 20–70 μm in diameter. The probe was a directional sensor with a half-band acceptance angle of 17–20°. Light measurements were made as the fiber optic probe was driven through plant tissue by a motorized micromanipulator, and the light that entered the fiber tip was piped to a spectroradiometer. By irradiating green leaf tissue of the succulent Crassula falcata L. with collimated light and inserting the probe from different directions, it was possible to measure light quality and quantity at different depths. Collimated light was scattered completely by the initial 1.0 mm of leaf tissue, which also greatly attenuated all light except the green and far-red. Light scatter contributed significantly to light quantity and had a pronounced spectral structure. Immediately beneath the irradiated surface the amount of light at 550 nm was 1.2 times that of the incident light. The light gradient declined rapidly to 0.5 times incident light at 1.4 mm depth. In contrast, the amount of light at 750 nm increased during the initial 0.5 mm to 2.9 times incident light and then declined linearly to 0.5 times incident light at the dark side of the leaf (4.5 mm). The implications of the magnitude of the contribution of light scatter to the light gradient is also discussed.
影响因子：
3.8
作者：
A. Profio;D. Doiron
通讯作者：
A. Profio;D. Doiron