Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
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DOI：
10.1186/s13567-016-0390-5
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发表时间：
2016-10-20
影响因子：
4.4
通讯作者：
Tchilian E
中科院分区：
文献类型：
--
作者：
Hemmink JD;Morgan SB;Aramouni M;Everett H;Salguero FJ;Canini L;Porter E;Chase-Topping M;Beck K;Loughlin RM;Carr BV;Brown IH;Bailey M;Woolhouse M;Brookes SM;Charleston B;Tchilian E
猪流感病毒感染是一个主要的养殖问题，造成相当大的经济损失，并构成人畜共患病的威胁。此外，猪是理解对流感病毒的免疫的极好模型，因为这是一种天然宿主病原体系统。在实验中，流感病毒通过鼻内、气管内滴注或气雾剂传递给猪，但几乎没有数据比较不同方法的结果。我们评估了鼻内或气雾剂将低或高剂量猪流感pdmH 1 N1病毒递送至猪呼吸道后的散毒模式、鼻拭子中的细胞因子应答和免疫应答，并将其与自然感染的接触猪中诱导的散毒模式和免疫应答进行比较。我们的数据表明，自然感染的接触诱导显着高的先天性和适应性免疫反应，虽然动物暴露于一个非常低的病毒剂量。在接触中，病毒脱落动力学缓慢且延长，与低剂量直接感染动物更相似。相比之下，鼻拭子中的细胞因子谱、接触者的抗体和细胞免疫应答更接近于高剂量直接接种动物的免疫应答。考虑这些差异对于疾病发病机制的研究和疫苗保护效力的评估是重要的。本文的在线版本（doi：10.1186/s13567-016-0390-5）包含补充材料，可供授权用户使用。
Influenza virus infection in pigs is a major farming problem, causing considerable economic loss and posing a zoonotic threat. In addition the pig is an excellent model for understanding immunity to influenza viruses as this is a natural host pathogen system. Experimentally, influenza virus is delivered to pigs intra-nasally, by intra-tracheal instillation or by aerosol, but there is little data comparing the outcome of different methods. We evaluated the shedding pattern, cytokine responses in nasal swabs and immune responses following delivery of low or high dose swine influenza pdmH1N1 virus to the respiratory tract of pigs intra-nasally or by aerosol and compared them to those induced in naturally infected contact pigs. Our data shows that natural infection by contact induces remarkably high innate and adaptive immune response, although the animals were exposed to a very low virus dose. In contacts, the kinetics of virus shedding were slow and prolonged and more similar to the low dose directly infected animals. In contrast the cytokine profile in nasal swabs, antibody and cellular immune responses of contacts more closely resemble immune responses in high dose directly inoculated animals. Consideration of these differences is important for studies of disease pathogenesis and assessment of vaccine protective efficacy. The online version of this article (doi:10.1186/s13567-016-0390-5) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Brookes SM;Núñez A;Choudhury B;Matrosovich M;Essen SC;Clifford D;Slomka MJ;Kuntz-Simon G;Garcon F;Nash B;Hanna A;Heegaard PM;Quéguiner S;Chiapponi C;Bublot M;Garcia JM;Gardner R;Foni E;Loeffen W;Larsen L;Van Reeth K;Banks J;Irvine RM;Brown IH
通讯作者：
Brown IH
影响因子：
1.8
作者：
Peres-Neto, PR;Jackson, DA;Somers, KM
通讯作者：
Somers, KM
影响因子：
3.7
作者：
De Vleeschauwer A;Atanasova K;Van Borm S;van den Berg T;Rasmussen TB;Uttenthal A;Van Reeth K
通讯作者：
Van Reeth K
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
158.5
作者：
Low, N.;Bavdekar, A.;Restrepo, A. M. Henao
通讯作者：
Restrepo, A. M. Henao