Conserved and Unique Putative Effectors Expressed in the Salivary Glands of Three Related Gall Midge Species.
Conserved and Unique Putative Effectors Expressed in the Salivary Glands of Three Related Gall Midge Species.
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DOI：
10.1093/jisesa/iey094
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发表时间：
2018-09-01
期刊：
影响因子：
--
通讯作者：
Chen MS
中科院分区：
文献类型：
--
作者：
Al-Jbory Z;El-Bouhssini M;Chen MS
麻叶瘿蚊属（双翅目：瘿蚊科）对小粮作物造成严重危害。麻叶蠓属有黑蝇（Mayetiola destructor Say）、大麦蠓（Mayetiola hordei Keiffer）和燕麦蠓（Mayetiola avenae Marchal）。这些物种的幼虫将唾液注入宿主组织以操纵植物。为了确定可能的效应蛋白，我们对大麦和燕麦蠓一龄幼虫分泌唾液腺蛋白（SSGPs）的转录本进行了转录组学分析，因为SSGPs是最有可能将效应蛋白传递到宿主组织的来源。从大麦蠓中共鉴定出178个ssgp编码基因，分为51个类群。取燕麦蠓194只，分为50组。一组内的预测蛋白具有高度保守的分泌信号肽，并且具有至少30%的氨基酸同源性。在大麦和燕麦蠓类中所鉴定的异基因中，约68%的异基因在三个种之间或两个种之间都是保守的。保守的ssgp包括属于SSGP-1、SSGP-4、SSGP-11和SSGP-71家族的成员。不同种类的瘿蚊家族成员之间以及不同种类的瘿蚊之间存在着非常规的保守模式，表明这些基因处于高选择压力下，这是效应基因的特征。每个物种特有的ssgp也被确定。这些保守的ssgp可能负责操纵宿主，因为三种瘿蚊对宿主植物产生相同的表型症状，而每种物种特有的ssgp可能负责不同的宿主特异性。
Species in the stem gall midge genus Mayetiola (Diptera: Cecidomyiidae) cause serious damage to small grain crops. Among Mayetiola species are Hessian fly (Mayetiola destructor Say), barley midge (Mayetiola hordei Keiffer), and oat midge (Mayetiola avenae Marchal). Larvae of these species inject saliva into host tissues to manipulate plants. To identify putative effectors, transcriptomic analyses were conducted on transcripts encoding secreted salivary gland proteins (SSGPs) from first instar larvae of the barley and oat midges, since SSGPs are the most likely source for effector proteins delivered into host tissues. From barley midge, 178 SSGP-encoding unigenes were identified, which were sorted into 51 groups. From oat midge, 194 were obtained and sorted into 50 groups. Predicted proteins within a group had a highly conserved secretion signal peptide and shared at least 30% amino acid identity. Among the identified unigenes from both barley and oat midges, ~68% are conserved either among the three species or between two of them. Conserved SSGPs included members belonging to SSGP-1, SSGP-4, SSGP-11, and SSGP-71 families. Unconventional conservation patterns exist among family members within a species and among different gall midges, indicating that these genes are under high selection pressure, a characteristic of effector genes. SSGPs that are unique to each species were also identified. Those conserved SSGPs may be responsible for host manipulation since the three gall midges produce identical phenotypic symptoms to host plants, whereas the SSGPs unique to each species may be responsible for different host specificity.
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影响因子：
5.3
作者：
Stuart, Jeff
通讯作者：
Stuart, Jeff
DOI：
10.1073/pnas.0709303105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Jiang, Rays H. Y.;Tripathy, Sucheta;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
9.8
作者：
Petre B;Kamoun S
通讯作者：
Kamoun S
影响因子：
2.6
作者：
Chen, MS;Fellers, JP;Liu, X
通讯作者：
Liu, X
影响因子：
9.2
作者：
Zhao, Chaoyang;Escalante, Lucia Navarro;Richards, Stephen
通讯作者：
Richards, Stephen