Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects.
Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects.
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DOI：
10.1093/humrep/der295
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Gianaroli L
中科院分区：
文献类型：
--
作者：
Magli MC;Montag M;Köster M;Muzi L;Geraedts J;Collins J;Goossens V;Handyside AH;Harper J;Repping S;Schmutzler A;Vesela K;Gianaroli L
本研究的目的是评估与极体（PB）活检相关的技术问题，这可能对微阵列比较基因组杂交分析的结果产生影响。此外，还对两种活检方法（机械和激光）进行了比较。第一个和第二个 PB（PB1 和 PB2）的活检是在两个不同的 IVF 中心通过机械或激光辅助活检进行的。通过全基因组扩增分别扩增PB。活检方法、机械或激光对成功活检卵母细胞的比例没有影响。特别是，对于 PB2，ICSI 后活检的时机与扩增效率直接相关。必须特别注意 PB2 活检的时间。机械辅助活检和激光辅助活检在诊断效率方面具有相同的性能。
The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency.
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影响因子：
6.7
作者：
Magli, M. Cristina;Lappi, Michela;Gianaroli, Luca
通讯作者：
Gianaroli, Luca
影响因子：
6.1
作者：
MUNNE, S;LEE, A;COHEN, J
通讯作者：
COHEN, J
影响因子：
6.7
作者：
Gianaroli, L;Magli, MC;Munné, S
通讯作者：
Munné, S
影响因子：
6.7
作者：
Magli, M. Cristina;Ferraretti, Anna P.;Gianaroli, Luca
通讯作者：
Gianaroli, Luca