Detection of subtle neurological alterations by the Catwalk XT gait analysis system.
Detection of subtle neurological alterations by the Catwalk XT gait analysis system.
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DOI：
10.1186/1743-0003-11-62
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发表时间：
2014-04-16
影响因子：
5.1
通讯作者：
Pan HC
中科院分区：
文献类型：
--
作者：
Chen YJ;Cheng FC;Sheu ML;Su HL;Chen CJ;Sheehan J;Pan HC
一种新版本的T型台XT系统被评估为一种基于更高的速度采样率检测非常细微的步态变化的工具；该系统还可以显示神经功能的微小变化。在这项研究中，我们评估了局部注射透明质酸干预坐骨神经损伤的神经学结果。使用T型台XT系统，我们寻找治疗组和未治疗组之间的差异以及同一组内作为时间函数的差异，以评估T型台XT系统在检测细微神经变化方面的能力。用血管夹夹闭左侧坐骨神经，造成36只SD大鼠周围神经损伤。实验动物随机分为两组：I组：挤压伤作为对照组，II组：挤压伤加透明质酸局部涂抹。定期对这些动物进行神经行为评估、组织形态评估和电生理检查。这些数据被检索以进行统计分析。损伤神经远端神经丝和S-100的密度在不同时间点的组间比较和7~28d的组内比较均有显著差异。神经结构构筑、轴突计数、髓鞘形成强度、电生理学和胶原沉积在两组之间显示出显著差异。组间比较7~28天的SFI和踝角差异有统计学意义，而7天和14天的SFI和踝角差异无统计学意义。在猫步XT分析中，强度、印记面积、站立持续时间和摆动持续时间在第7天、第14天、第21天和第28天都显示出明显的差异，而在第7天和第14天与T型台7测试中没有显著差异。此外，两个版本在步序和规律性指数上也没有显著差异。早在挤压伤后7天，透明质酸就促进了神经再生。这种细微的神经变化可以通过T型台XT步态分析来检测，但不能通过SFI、脚踝角度或T型台7方法来检测。
A new version of the CatWalk XT system was evaluated as a tool for detecting very subtle alteration in gait based on higher speed sample rate; the system could also demonstrate minor changes in neurological function. In this study, we evaluated the neurological outcome of sciatic nerve injury intervened by local injection of hyaluronic acid. Using the CatWalk XT system, we looked for differences between treated and untreated groups and differences within the same group as a function of time so as to assess the power of the Catwalk XT system for detecting subtle neurological change. Peripheral nerve injury was induced in 36 Sprague–Dawley rats by crushing the left sciatic nerve using a vessel clamp. The animals were randomized into one of two groups: Group I: crush injury as the control; Group II: crush injury and local application with hyaluronic acid. These animals were subjected to neurobehavior assessment, histomorphology evaluation, and electrophysiology study periodically. These data were retrieved for statistical analysis. The density of neurofilament and S-100 over the distal end of crushed nerve showed significant differences either in inter-group comparison at various time points or intra-group comparison from 7 to 28 days. Neuronal structure architecture, axon counts, intensity of myelination, electrophysiology, and collagen deposition demonstrate significant differences between the two groups. There was significant difference of SFI and angle of ankle in inter- group analysis from 7 to 28 days, but there were no significant differences in SFI and angle of ankle at time points of 7 and 14 days. In the Cat Walk XT analysis, the intensity, print area, stance duration, and swing duration all showed detectable differences at 7, 14, 21, and 28 days, whereas there were no significant difference at 7 and 14 days with CatWalk 7 testing. In addition, there were no significant differences of step sequence or regularity index between the two versions. Hyaluronic acid augmented nerve regeneration as early as 7 days after crush injury. This subtle neurological alteration could be detected through the CatWalk XT gait analysis but not the SFI, angle of ankle, or CatWalk 7 methods.
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影响因子：
4.2
作者：
Hamers, FPT;Koopmans, GC;Joosten, EAJ
通讯作者：
Joosten, EAJ
影响因子：
3
作者：
WALKER, JL;EVANS, JM;SISKEN, BF
通讯作者：
SISKEN, BF
影响因子：
4.4
作者：
Pan, Hung-Chuan;Chen, Chung-Jung;Wang, Yeou-Chih
通讯作者：
Wang, Yeou-Chih
DOI：
10.1007/bf01188409
发表时间：
1990-06-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
MATA, M;ALESSI, D;FINK, DJ
通讯作者：
FINK, DJ
影响因子：
5.3
作者：
DEMEDINACELI, L;FREED, WJ;WYATT, RJ
通讯作者：
WYATT, RJ