Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice.
Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice.
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DOI：
10.1016/j.stemcr.2014.03.011
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发表时间：
2014-05-06
影响因子：
5.9
通讯作者：
Takahashi, Masayo
中科院分区：
文献类型：
--
作者：
Assawachananont, Juthaporn;Mandai, Michiko;Okamoto, Satoshi;Yamada, Chikako;Eiraku, Mototsugu;Yonemura, Shigenobu;Sasai, Yoshiki;Takahashi, Masayo
在这篇文章中，我们表明小鼠胚胎干细胞或诱导多能干细胞衍生的3D视网膜组织即使在缺乏ONL的晚期视网膜变性模型（rd 1）中也发育出了具有完整内外段的结构化外核层（ONL）。我们还通过免疫组织化学观察了宿主-移植物突触连接。本研究为视网膜薄片移植治疗晚期视网膜变性疾病提供了“概念验证”。使用视黄酸受体拮抗剂ESC/iPSC从ESC/iPSC形成3D视网膜在rd 1宿主小鼠中视网膜发育出结构化的光感受器层移植的光感受器形成完整的内节和外节移植的光感受器与宿主双极细胞在形态学上形成突触在本文中，Mandai及其同事表明，小鼠胚胎干细胞或诱导多能干细胞衍生的3D视网膜组织发育出结构化的外核层（ONL），甚至在缺乏ONL的晚期视网膜变性模型（rd 1）中也是如此。他们还通过免疫组织化学观察了宿主-移植物突触连接。本研究为视网膜薄片移植治疗晚期视网膜变性疾病提供了“概念验证”。
In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a “proof of concept” for retinal sheet transplantation therapy for advanced retinal degenerative diseases. 3D retina was formed from ESCs/iPSCs using retinoic acid receptor antagonist ESC/iPSC retina developed a structured layer of photoreceptors in rd1 host mice Transplanted photoreceptors formed complete inner and outer segments Transplanted photoreceptors morphologically made synapses with host bipolar cells In this article, Mandai and colleagues show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. They also observed host-graft synaptic connections by immunohistochemistry. This study provides a “proof of concept” for retinal sheet transplantation therapy for advanced retinal degenerative diseases.
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影响因子：
64.8
作者：
Pearson, R. A.;Barber, A. C.;Rizzi, M.;Hippert, C.;Xue, T.;West, E. L.;Duran, Y.;Smith, A. J.;Chuang, J. Z.;Azam, S. A.;Luhmann, U. F. O.;Benucci, A.;Sung, C. H.;Bainbridge, J. W.;Carandini, M.;Yau, K. -W.;Sowden, J. C.;Ali, R. R.
通讯作者：
Ali, R. R.
DOI：
10.1002/stem.1372
发表时间：
2013-06
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Homma K;Okamoto S;Mandai M;Gotoh N;Rajasimha HK;Chang YS;Chen S;Li W;Cogliati T;Swaroop A;Takahashi M
通讯作者：
Takahashi M
影响因子：
--
作者：
Jacobson, Samuel G.;Cideciyan, Artur V.;Ratnakaram, Ramakrishna;Heon, Elise;Schwartz, Sharon B.;Roman, Alejandro J.;Peden, Marc C.;Aleman, Tomas S.;Boye, Sanford L.;Sumaroka, Alexander;Conlon, Thomas J.;Calcedo, Roberto;Pang, Ji-Jing;Erger, Kirsten E.;Olivares, Melani B.;Mullins, Cristina L.;Swider, Malgorzata;Kaushal, Shalesh;Feuer, William J.;Iannaccone, Alessandro;Fishman, Gerald A.;Stone, Edwin M.;Byrne, Barry J.;Hauswirth, William W.
通讯作者：
Hauswirth, William W.
影响因子：
3.4
作者：
Arai, S;Thomas, BB;Sadda, SR
通讯作者：
Sadda, SR
DOI：
10.1073/pnas.0508214103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Akimoto, M;Cheng, H;Swaroop, A
通讯作者：
Swaroop, A