Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri.
Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri.
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接种或接种植物乳杆菌或布氏乳杆菌的青贮紫花苜蓿的代谢组和细菌群落动态分析
DOI：
10.1038/s41598-017-18348-0
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发表时间：
2018-01-10
影响因子：
4.6
通讯作者：
Yang FY
中科院分区：
文献类型：
--
作者：
Guo XS;Ke WC;Ding WR;Ding LM;Xu DM;Wang WW;Zhang P;Yang FY
使用气相色谱质谱和PacBio单分子实时测序技术（SMRT），我们分析了详细的代谢谱和微生物群落动态参与青贮苜蓿（苜蓿）接种或不接种同型发酵植物乳杆菌或异型发酵布氏乳杆菌。结果表明，青贮苜蓿中存在280种物质和102种不同的代谢产物。接种L. Buchneri使青贮苜蓿中4-氨基丁酸、部分游离氨基酸和多元醇的含量显著增加，而L.植物接种的青贮饲料。在对照组和两种接种剂处理的青贮饲料中观察到完全不同的微生物植物群及其在青贮过程中的演替。接种L. plantarum或L. Buchneri以非常不同的方式改变青贮饲料的微生物组成动力学。我们的研究表明，代谢组学分析提供了一个深刻的洞察青贮饲料中的代谢产物。此外，PacBio SMRT方法揭示了青贮过程中的微生物组成及其在物种水平上的演替。这提供了关于青贮饲料形成的微生物过程的信息，并可能有助于基于目标的调控方法，以实现高质量的青贮饲料生产。
Using gas chromatography mass spectrometry and the PacBio single molecule with real-time sequencing technology (SMRT), we analyzed the detailed metabolomic profiles and microbial community dynamics involved in ensiled Medicago sativa (alfalfa) inoculated without or with the homofermenter Lactobacillus plantarum or heterofermenter Lactobacillus buchneri. Our results revealed that 280 substances and 102 different metabolites were present in ensiled alfalfa. Inoculation of L. buchneri led to remarkable up-accumulation in concentrations of 4-aminobutyric acid, some free amino acids, and polyols in ensiled alfalfa, whereas considerable down-accumulation in cadaverine and succinic acid were observed in L. plantarum-inoculated silages. Completely different microbial flora and their successions during ensiling were observed in the control and two types of inoculant-treated silages. Inoculation of the L. plantarum or L. buchneri alters the microbial composition dynamics of the ensiled forage in very different manners. Our study demonstrates that metabolomic profiling analysis provides a deep insight in metabolites in silage. Moreover, the PacBio SMRT method revealed the microbial composition and its succession during the ensiling process at the species level. This provides information regarding the microbial processes underlying silage formation and may contribute to target-based regulation methods to achieve high-quality silage production.
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影响因子：
2.4
作者：
Li, Y.;Nishino, N.
通讯作者：
Nishino, N.
影响因子：
4.4
作者：
Ennahar, S;Cai, YM;Fujita, Y
通讯作者：
Fujita, Y
影响因子：
4
作者：
Arasu, M. Valan;Jung, M. -W.;Choi, K. -C.
通讯作者：
Choi, K. -C.
影响因子：
4
作者：
McEniry, J.;O'Kiely, P.;Doyle, E. M.
通讯作者：
Doyle, E. M.
影响因子：
3.5
作者：
Schmidt, R. J.;Hu, W.;Kung, L., Jr.
通讯作者：
Kung, L., Jr.