The RNA chaperone Hfq is essential for the virulence of Salmonella typhimurium.
The RNA chaperone Hfq is essential for the virulence of Salmonella typhimurium.
复制标题
RNA伴侣HFQ对于沙门氏菌伤寒的毒力至关重要。
DOI：
10.1111/j.1365-2958.2006.05489.x
复制
发表时间：
2007-01
影响因子：
3.6
通讯作者：
Vogel J
中科院分区：
文献类型：
--
作者：
Sittka A;Pfeiffer V;Tedin K;Vogel J
RNA分子伴侣Hfq在细菌生理学中发挥着多种作用，超出了其作为Qβ RNA噬菌体复制所需的宿主因子的原始作用。在这项研究中，我们表明，Hfq参与的表达和分泌的毒力因子的兼性细胞内病原体，鼠伤寒沙门氏菌。沙门氏菌hfq缺失菌株在口服和腹膜内感染后在小鼠中高度减毒，并且在体外显示上皮细胞侵袭的严重缺陷以及上皮细胞和巨噬细胞的生长缺陷。令人惊讶的是，我们发现，这些表型在很大程度上是独立的，以前报道的要求Hfq表达的固定相西格玛因子，RpoS。我们的研究结果暗示Hfq作为毒力的多个方面的关键调节剂，包括调节运动性和外膜蛋白（OmpD）的表达，除了入侵和细胞内生长。这些多效性的影响，建议涉及一个网络的监管小的非编码RNA，将Hfq在沙门氏菌的毒力基因表达的转录后调控的中心。此外，hfq突变似乎引起RpoE介导的包膜应激反应的慢性激活，这可能是由于膜蛋白表达的误调节。
The RNA chaperone, Hfq, plays a diverse role in bacterial physiology beyond its original role as a host factor required for replication of Qβ RNA bacteriophage. In this study, we show that Hfq is involved in the expression and secretion of virulence factors in the facultative intracellular pathogen, Salmonella typhimurium. A Salmonella hfq deletion strain is highly attenuated in mice after both oral and intraperitoneal infection, and shows a severe defect in invasion of epithelial cells and a growth defect in both epithelial cells and macrophages in vitro. Surprisingly, we find that these phenotypes are largely independent of the previously reported requirement of Hfq for expression of the stationary phase sigma factor, RpoS. Our results implicate Hfq as a key regulator of multiple aspects of virulence including regulation of motility and outer membrane protein (OmpD) expression in addition to invasion and intracellular growth. These pleiotropic effects are suggested to involve a network of regulatory small non-coding RNAs, placing Hfq at the centre of post-transcriptional regulation of virulence gene expression in Salmonella. In addition, the hfq mutation appears to cause a chronic activation of the RpoE-mediated envelope stress response which is likely due to a misregulation of membrane protein expression.
登录
查看更多内容
影响因子：
2.8
作者：
Hara-Kaonga, B;Pistole, TG
通讯作者：
Pistole, TG
影响因子：
3.6
作者：
Darwin, KH;Miller, VL
通讯作者：
Miller, VL
影响因子：
3.2
作者：
Christiansen, JK;Larsen, MH;Kallipolitis, BH
通讯作者：
Kallipolitis, BH
影响因子：
2.9
作者：
Doherty, NS;Littman, BH;Anderson, NL
通讯作者：
Anderson, NL
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL