Detection of plant volatiles after leaf wounding and darkening by proton transfer reaction "time-of-flight" mass spectrometry (PTR-TOF).
Detection of plant volatiles after leaf wounding and darkening by proton transfer reaction "time-of-flight" mass spectrometry (PTR-TOF).
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DOI：
10.1371/journal.pone.0020419
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hansel A
中科院分区：
文献类型：
--
作者：
Brilli F;Ruuskanen TM;Schnitzhofer R;Müller M;Breitenlechner M;Bittner V;Wohlfahrt G;Loreto F;Hansel A
采用质子转移反应飞行时间(PTR-TOF)质谱仪对叶片伤害和变暗诱导的生物挥发性有机化合物(BVOCs)进行了检测。PTR-TOF测量明确地捕捉到了伤害和变暗后绿叶挥发物(GLV)和乙醛的大量排放的动力学。GLV的释放与损伤的程度相关，因此证实是机械损伤的一个很好的指标。在伤害后，也检测到了不排放异戊二烯的植物物种的甲醇、C5化合物和异戊二烯的瞬时排放。在异戊二烯释放较强的白杨中，由于剪叶的光合作用抑制，光依赖性异戊二烯的释放持续了数小时，甚至增强了数小时。因此，异戊二烯的排放可以从光合作用中分离出来，甚至在砍掉树叶或树枝之后也可能发生，例如，通过农业实践或由于非生物和生物胁迫。这一观测结果可能对评估大气中异戊二烯的来源和预算以及对流层化学的后果有重要影响。
Proton transfer reaction-time of flight (PTR-TOF) mass spectrometry was used to improve detection of biogenic volatiles organic compounds (BVOCs) induced by leaf wounding and darkening. PTR-TOF measurements unambiguously captured the kinetic of the large emissions of green leaf volatiles (GLVs) and acetaldehyde after wounding and darkening. GLVs emission correlated with the extent of wounding, thus confirming to be an excellent indicator of mechanical damage. Transient emissions of methanol, C5 compounds and isoprene from plant species that do not emit isoprene constitutively were also detected after wounding. In the strong isoprene-emitter Populus alba, light-dependent isoprene emission was sustained and even enhanced for hours after photosynthesis inhibition due to leaf cutting. Thus isoprene emission can uncouple from photosynthesis and may occur even after cutting leaves or branches, e.g., by agricultural practices or because of abiotic and biotic stresses. This observation may have important implications for assessments of isoprene sources and budget in the atmosphere, and consequences for tropospheric chemistry.
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影响因子：
7.3
作者：
Beauchamp, J;Wisthaler, A;Wildt, J
通讯作者：
Wildt, J
影响因子：
9.4
作者：
Brilli, Federico;Barta, Csengele;Centritto, Mauro
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
SLUSARENKO, AJ
DOI：
10.1016/j.jasms.2010.02.006
发表时间：
2010-06-01
影响因子：
3.2
作者：
Graus, Martin;Mueller, Markus;Hansel, Armin
通讯作者：
Hansel, Armin