Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene.
Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene.
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用于囊性纤维化基因克隆的 7 号染色体上 DNA 标记的鉴定和区域定位。
DOI：
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发表时间：
1988
影响因子：
9.8
通讯作者：
Lap
中科院分区：
文献类型：
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作者：
J. Rommens;S. Zengerling;Julie E. Burns;Georg Melmer;B. Kerem;N. Plavsic;M. Zsiga;D. Kennedy;D. Markiewicz;R. Rozmahel;John R. Riordan;Manuel Buchwald;Lap
为了便于定位囊性纤维化基因(CF)并分离相应的基因，我们在7q31-q32区域筛选了7号染色体特异性DNA标记的流动排序文库。从文库中选择独特的(单拷贝)DNA片段，并用于与一组包含人类7号染色体不同部分的体细胞杂交体和具有该染色体缺失的患者细胞系的杂交分析。共鉴定出258个7号染色体特异的单拷贝DNA片段，其中大部分定位于亚区。其中53个与7q31-q32区域的DNA序列相对应。家系和物理作图研究表明，D7S122和D7S340这两个DNA标记与CF紧密连锁。数据还表明，D7S122和D7S340位于MET和D7S8之间，这两个遗传标记位于Cf的相反两侧。因此，这项研究重申了在染色体定位的基础上接近疾病部位的一般策略。
To facilitate mapping of the cystic fibrosis locus (CF) and to isolate the corresponding gene, we have screened a flow-sorted chromosome 7-specific library for additional DNA markers in the 7q31-q32 region. Unique ("single-copy") DNA segments were selected from the library and used in hybridization analysis with a panel of somatic cell hybrids containing various portions of human chromosome 7 and patient cell lines with deletion of this chromosome. A total of 258 chromosome 7-specific single-copy DNA segments were identified, and most of them localized to subregions. Fifty three of these corresponded to DNA sequences in the 7q31-q32 region. Family and physical mapping studies showed that two of the DNA markers, D7S122 and D7S340, are in close linkage with CF. The data also showed that D7S122 and D7S340 map between MET and D7S8, the two genetic markers known to be on opposite sides of CF. The study thus reaffirms the general strategy in approaching a disease locus on the basis of chromosome location.
影响因子：
56.9
作者：
LEPPERT, M;DOBBS, M;WHITE, R
通讯作者：
WHITE, R
影响因子：
56.9
作者：
STGEORGEHYSLOP, PH;TANZI, RE;GUSELLA, JF
通讯作者：
GUSELLA, JF