Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection.
Expression of γH2AX in various gastric pathologies and its association with Helicobacter pylori infection.
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DOI：
10.3892/ol.2013.1693
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发表时间：
2014-01
期刊：
影响因子：
2.9
通讯作者：
Lu NH
中科院分区：
文献类型：
--
作者：
Xie C;Xu LY;Yang Z;Cao XM;Li W;Lu NH
Ser139位H_2AX的磷酸化(γH_2AX)是DNA双链断裂的生物标志物。本研究旨在探讨γ-H_2AX水平与胃组织病理及幽门螺杆菌感染的关系。胃活检标本来自幽门螺杆菌阴性和阳性患者302例，包括慢性胃炎(CG)、肠化生(IM)、异型增生(Dys)和胃癌(GC)。从5个胃上皮细胞系和10例配对的胃癌及癌旁正常组织中提取蛋白质。免疫组织化学和免疫印迹法检测胃组织中DNADSBs生物标志物γH 2AX的表达。γ、H_2AX在胃癌组织中的表达随病理分期的不同而逐渐升高，而在胃癌组织中的表达略有降低。幽门螺杆菌感染与γH_2AX表达增加、IM和Dys有关。γ-H_2AX在胃癌中的过表达与肿瘤的部位、大体形态、分化程度、侵袭深度、TNM分期和有无淋巴结转移有关。提示DSB可能是胃癌发生的早期分子事件，可能与幽门螺杆菌感染有关。此外，免疫组织化学γ-H_2AX染色与多种临床病理特征相关。γ-H_2AX的表达可作为胃癌诊断和预后判断的一个有价值的生物标志物。
Phosphorylation of H2AX at Ser 139 (γH2AX) is a biomarker of DNA double-strand breaks (DSBs). The present study aimed to explore the association between γH2AX levels and gastric pathology and Helicobacter pylori (H. pylori) infection. Gastric biopsies were obtained from 302 H. pylori-negative and -positive patients, including those with chronic gastritis (CG), intestinal metaplasia (IM), dysplasia (Dys) and gastric cancer (GC). Proteins were extracted from five gastric epithelial cell lines and from 10 specimens of matched GC and adjacent normal tissues. The expression of γH2AX, a biomarker for the detection of DNA DSBs, in gastric tissues was detected by immunohistochemistry and western blotting. The expression of γH2AX progressively increased in tissues according to pathological stage from CG to Dys, but was slightly decreased in GC. H. pylori infection was associated with increased γH2AX expression, IM and Dys. Overexpression of γH2AX in GC was found to correlate with tumor location, gross appearance, differentiation, depth of invasion, TNM stage and lymph node metastasis. The results indicated that DSBs appear to be an early molecular event in gastric carcinogenesis, which may be associated with H. pylori infection. Moreover, immunohistochemical staining of γH2AX was found to correlate with a number of clinicopathological characteristics. The expression of γH2AX may serve as a valuable biomarker for the diagnosis and progression of GC.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
5.7
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影响因子：
6.4
作者：
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通讯作者：
Parkin, Donald Maxwell