Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier.
Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier.
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灌注梭状芽胞杆菌肠毒素片段从紧密连接链中去除特定的克劳丁：直接参与Claudins在紧密连接屏障中的证据。
DOI：
10.1083/jcb.147.1.195
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发表时间：
1999-10-04
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Sonoda N;Furuse M;Sasaki H;Yonemura S;Katahira J;Horiguchi Y;Tsukita S
Claudins组成一个多基因家族，构成紧密连接(TJ)链。产气荚膜梭菌肠毒素是一种单一的∼35-kD多肽，能与Claudin-3/RVP1和Claudin-4/Cpe-R在其COOH端特异性结合。我们检测了CPE的羧基末端半片段(C-CPE)对表达Claudin-1~-4的L细胞(C1L~C4L)以及表达Claudin-1和-4的MDCK I细胞TJs的影响。C-CPE与claudin-3、-4高亲和力结合，但不与claudin-1、-2结合。在C-CPE存在下，C3L细胞中重组的TJ链逐渐解体并从细胞表面消失。在与C-CPE孵育的MDCK I细胞中，Claudin-4被选择性地从TJ中去除，同时伴随着它的降解。C-CPE孵育4h后，TJ链解体，TJ链数目减少，TJ链网络复杂程度明显降低。C-CPE以剂量依赖的方式下调MDCK I细胞的TJ屏障(TER和细胞旁通透性)，这与这些形态变化的时间进程相一致。这些发现为Claudins直接参与TJ的屏障功能提供了证据。
Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single ∼35-kD polypeptide, was reported to specifically bind to claudin-3/RVP1 and claudin-4/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.
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影响因子：
--
作者：
BRIEHL, MM;MIESFELD, RL
通讯作者：
MIESFELD, RL
通过表达突变型紧密连接膜蛋白，细胞旁通透性和跨上皮电阻的功能性分离以及顶端-基底外侧膜内扩散屏障的破坏。
影响因子：
7.8
作者：
Balda, M S;Whitney, J A;Flores, C;Gonzalez, S;Cereijido, M;Matter, K
通讯作者：
Matter, K
影响因子：
3.1
作者：
HORIGUCHI, Y;AKAI, T;SAKAGUCHI, G
通讯作者：
SAKAGUCHI, G
单个基因产物Claudin -1或-2，在成纤维细胞中重构紧密结合的连接链和新兵。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Fujimoto, K;Tsukita, S
通讯作者：
Tsukita, S
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S