15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell.
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell.
复制标题
15-氧代二十碳四烯酸介导单核细胞与内皮细胞的粘附
DOI：
10.1186/s12944-017-0518-2
复制
发表时间：
2017-07-12
影响因子：
4.5
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Ma G;Pan B;Ren S;Guo C;Guo Y;Wei L;Zheng L;Chen B
BackgroundA great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis.MethodsThe 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry andImmunohistochemistry.ResultsIncreased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion.ConclusionsThis is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC.
登录
查看更多内容
影响因子：
3.7
作者：
Pan B;Ma Y;Ren H;He Y;Wang Y;Lv X;Liu D;Ji L;Yu B;Wang Y;Chen YE;Pennathur S;Smith JD;Liu G;Zheng L
通讯作者：
Zheng L
DOI：
10.1161/atvbaha.116.308014
发表时间：
2016-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Herbin O;Regelmann AG;Ramkhelawon B;Weinstein EG;Moore KJ;Alexandropoulos K
通讯作者：
Alexandropoulos K
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
9.1
作者：
Matsuzawa, Yasushi;Guddeti, Raviteja R.;Kwon, Taek-Geun;Lerman, Lilach O.;Lerman, Amir
通讯作者：
Lerman, Amir
影响因子：
20.1
作者：
Geraldes P;King GL
通讯作者：
King GL