Prevotella histicola Protects From Arthritis by Expansion of Allobaculum and Augmenting Butyrate Production in Humanized Mice.
Prevotella histicola Protects From Arthritis by Expansion of Allobaculum and Augmenting Butyrate Production in Humanized Mice.
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DOI：
10.3389/fimmu.2021.609644
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发表时间：
2021
影响因子：
7.3
通讯作者：
Taneja V
中科院分区：
文献类型：
--
作者：
Balakrishnan B;Luckey D;Bodhke R;Chen J;Marietta E;Jeraldo P;Murray J;Taneja V
细菌疗法是治疗风湿性关节炎等自身免疫性疾病的新兴选择。P. histicola MCI 001是一种这样的治疗性细菌，其已被证明在动物模型中治疗自身免疫性疾病，如RA和多发性硬化症[MS]。本研究表征了从人十二指肠活组织检查中分离的组织毕赤酵母MCI 001，并在HLA-DQ 8.AEo转基因小鼠中使用胶原诱导的关节炎模型的纵向研究中评估了其对肠道微生物和代谢谱的影响。尽管与组织疫霉的模式菌株DSM 19854密切相关，但组织疫霉MCI 001在利用甘油方面不同。在培养中，P. histicola MCI 001产生维生素，如生物素和叶酸，并参与消化复杂的碳水化合物和产生乙酸。对MCI 001治疗后关节炎小鼠肠道菌群的纵向追踪表明，MCI 001治疗后，关节炎引起的肠道菌群失调部分恢复到未治疗小鼠的水平。分类水平的分析表明，在MCI 001治疗的关节炎小鼠肠道Allobaculum属的扩展。在用P. histicola MCI 001处理后实现的生态良好也反映在短链脂肪酸[SCFA]的产量增加上。目前的研究表明，用P. histicola MCI 001处理通过增加简单碳水化合物和乙酸盐的可用性导致Allobaculum的扩张。微生物谱和代谢物如丁酸盐的恢复诱导免疫和肠道稳态。
Bacterial therapeutics are the emergent alternatives in treating autoimmune diseases such as Rheumatoid Arthritis [RA]. P. histicola MCI 001 is one such therapeutic bacterium that has been proven to treat autoimmune diseases such as RA and multiple sclerosis [MS] in animal models. The present study characterized P. histicola MCI 001 isolated from a human duodenal biopsy, and evaluated its impact on the gut microbial and metabolic profile in a longitudinal study using the collagen-induced arthritis model in HLA-DQ8.AEo transgenic mice. P. histicola MCI 001 though closely related to the type strain of P. histicola, DSM 19854, differed in utilizing glycerol. In culture, P. histicola MCI 001 produced vitamins such as biotin and folate, and was involved in digesting complex carbohydrates and production of acetate. Colonization study showed that duodenum was the predominant niche for the gavaged MCI 001. A longitudinal follow-up of gut microbial profile in arthritic mice treated with MCI 001 suggested that dysbiosis caused due to arthritis was partially restored to the profile of naïve mice after treatment. A taxon-level analysis suggested an expansion of intestinal genus Allobaculum in MCI001 treated arthritic mice. Eubiosis achieved post treatment with P. histicola MCI 001 was also reflected in the increased production of short-chain fatty acids [SCFAs]. Present study suggests that the treatment with P. histicola MCI 001 leads to an expansion of Allobaculum by increasing the availability of simple carbohydrates and acetate. Restoration of microbial profile and metabolites like butyrate induce immune and gut homeostasis.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
5.2
作者：
Cani PD;de Vos WM
通讯作者：
de Vos WM
影响因子：
--
作者：
Herrmann E;Young W;Rosendale D;Reichert-Grimm V;Riedel CU;Conrad R;Egert M
通讯作者：
Egert M
DOI：
10.1038/ismej.2014.45
发表时间：
2014-10
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
15.5
作者：
Gorvitovskaia A;Holmes SP;Huse SM
通讯作者：
Huse SM