High-Quality Queens Produce High-Quality Offspring Queens.
High-Quality Queens Produce High-Quality Offspring Queens.
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高质量的女王产生高质量的后代女王。
DOI：
10.3390/insects13050486
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发表时间：
2022-05-23
期刊：
影响因子：
3
通讯作者：
Wu, Xiaobo
中科院分区：
文献类型：
--
作者：
Yu, Longtao;Shi, Xinxin;He, Xujiang;Zeng, Zhijiang;Yan, Weiyu;Wu, Xiaobo
母体效应是动物调整后代质量的明智策略。蜂后可以调整对雌性后代的投资，以最大限度地利用社会资源，这些不同的投资最终可能导致不同的后代质量。然而，在人工育王中，母畜对育王质量的影响一直被忽视。为了阐明母性对饲养蜂王表型和遗传改变的影响，我们研究了由母性影响引起的卵源对饲养蜂王及其后代蜂王发育的影响。本研究结果对提高蜂王饲养质量，促进养蜂业的发展具有一定的指导意义。蜜蜂，而不是后方皇后与鸡蛋和幼虫从工人细胞，更喜欢后方新的皇后与鸡蛋形成皇后细胞，如果有。这可能是蜂群长期进化的结果。然而，这种现象的确切机制尚不清楚。在这项研究中，用来自蜂王细胞的卵（F1-QE）、来自工蜂细胞的卵（F1-WE）和来自工蜂细胞的两日龄幼虫（F1-2L）饲养蜂王。测定并比较了F1代蜂王的生理指标和发育相关基因（六角蛋白（Hex 110，Hex 70 b）、转铁蛋白（Trf）和卵黄蛋白原（Vg））的表达。用F1代蜂王的1日龄幼虫饲养F2代蜂王，并对饲养的F2代蜂王的体重和卵巢管计数进行了测定。同时，进一步研究了F2代蚁后发育和生殖相关基因（Hex 110、Hex 70 b、Trf、Vg和保幼激素（Jh））以及免疫解毒相关基因（Hyataecin、Abeacin和CytP 450）的表达。结果表明，F1-QE蜂王的各项生理指标最高，Hex 110和Trf的表达量最高，而Hex 70 b和Vg的表达量在3组F1蜂王中差异不显著。另外，F2-QE的育后质量最高，发育、生殖、免疫解毒基因的表达水平最高。结果表明，高质量蜂王所培育的后代蜂王质量也较高。这些发现为培育高质量蜂王的方法提供了信息，并强调了高质量蜂王对后代群体的生长和生存至关重要。
Maternal effects are a wise strategy for animals to adjust their offspring quality. Honey bee queens can adjust their investments in their female offspring to maximize the use of social resources, and these different investments may eventually lead to differing offspring quality. However, maternal effects on the quality of reared queens in artificial queen rearing has been ignored. To elucidate the maternal effects on the phenotypic and genetic alterations of reared queens, we examined the effect of ova sources caused by maternal effects on the development of reared queens and their offspring queens. Our results will shed light on improving the quality of reared queens and promoting the development of beekeeping. Honey bees, rather than rear queens with eggs and larvae from worker cells, prefer to rear new queens with eggs form queen cells, if available. This may be a result of long-term evolutionary process for honey bee colonies. However, the exact mechanism of this phenomenon is unclear. In this study, queens were reared with eggs from queen cells (F1-QE), eggs from worker cells (F1-WE), and two-day-old larvae from worker cells (F1-2L). Physiological indexes and the expression of the development-related genes ((Hexamerin (Hex110, Hex70b), Transferrin (Trf), and Vitellogenin (Vg)) of reared F1 generation queens were measured and compared. Furthermore, F2 generation queens were reared with one-day-old larvae from F1 queens, and the weight and ovariole count of reared F2 generation daughter queens were examined. Meanwhile, the expression of the development- and reproduction-related genes (Hex110, Hex70b, Trf, Vg, and Juvenile Hormone (Jh)) and immune detoxication-related genes (Hymenoptaecin, Abeacin, and CytP450) of reared F2 queens were further explored. We found that the F1-QE queens had the highest physiological indexes and higher Hex110 and Trf expression levels, while no significant difference was found in the expression of Hex70b and Vg among the three groups of F1 queens. In addition, the reared queens of F2-QE had the highest quality, with the highest development, reproduction, immune-detoxication genes’ expression levels. Our results revealed that the quality of reared offspring queens from high-quality mother queens was also high. These findings inform methods for rearing high-quality queens and highlight that a high-quality queen is essential for offspring colony growth and survival.
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影响因子：
3
作者：
Amiri E;Strand MK;Rueppell O;Tarpy DR
通讯作者：
Tarpy DR
影响因子：
2.2
作者：
den Boer, Susanne P. A.;Boomsma, Jacobus J.;Baer, Boris
通讯作者：
Baer, Boris
影响因子：
1.3
作者：
PASSERA, L
通讯作者：
PASSERA, L
影响因子：
2.2
作者：
Martins, Juliana Ramos;Franco Nunes, Francis Morais;Gentile Bitondi, Marcia Maria
通讯作者：
Gentile Bitondi, Marcia Maria
影响因子：
2.1
作者：
Amiri, Esmaeil;Le, Kevin;Rueppell, Olav
通讯作者：
Rueppell, Olav