Prognostic significance of epithelial-mesenchymal transition-related markers in extrahepatic cholangiocarcinoma: comprehensive immunohistochemical study using a tissue microarray.
Prognostic significance of epithelial-mesenchymal transition-related markers in extrahepatic cholangiocarcinoma: comprehensive immunohistochemical study using a tissue microarray.
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DOI：
10.1038/bjc.2014.415
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发表时间：
2014-09-23
影响因子：
8.8
通讯作者：
Matsuno, Y.
中科院分区：
文献类型：
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作者：
Nitta, T.;Mitsuhashi, T.;Hatanaka, Y.;Miyamoto, M.;Oba, K.;Tsuchikawa, T.;Suzuki, Y.;Hatanaka, K. C.;Hirano, S.;Matsuno, Y.
关键词：
上皮-间充质转化（EMT）的特征是细胞间粘附的丧失和间充质表型的获得。上皮-间质转化被认为发生在各种发育过程和癌症进展中。“Cadherin开关”是指细胞向表达不同的Cadherin跨膜蛋白的过程，通常是指从E-cadherin表达到N-cadherin表达的转换，是EMT的一个方面，对肿瘤侵袭/转移有深远的影响。本研究旨在探讨emt相关蛋白及钙粘蛋白开关在肝外胆管癌（EHCC）中的临床病理意义。我们利用组织微阵列免疫组化技术研究了EHCC患者（n=117） 12种emt相关蛋白的表达改变与临床结果之间的关系。单因素和多因素分析显示，除了N分类（P=0.0420）外，E-cadherin （P=0.0208）、N-cadherin （P=0.0038）和S100A4 （P=0.0157）的表达均是独立且显著的预后因素。我们还证明了钙粘蛋白开关与EHCC患者的不良预后独立相关（P=0.0143）。这些结果可能为选择需要辅助治疗和严格监测的EHCC患者提供新的信息。
Epithelial–mesenchymal transition (EMT) is characterised by the loss of cell-to-cell adhesion and gaining of mesenchymal phenotypes. Epithelial–mesenchymal transition is proposed to occur in various developmental processes and cancer progression. ‘Cadherin switch', a process in which cells shift to express different isoforms of the cadherin transmembrane protein and usually refers to a switch from the expression of E-cadherin to N-cadherin, is one aspect of EMT and can have a profound effect on tumour invasion/metastasis. The aim of this study was to investigate the clinicopathological significance of EMT-related proteins and cadherin switch in extrahepatic cholangiocarcinoma (EHCC). We investigated the association between altered expression of 12 EMT-related proteins and clinical outcomes in patients with EHCC (n=117) using immunohistochemistry on tissue microarrays. Univariate and multivariate analyses revealed that, in addition to N classification (P=0.0420), the expression of E-cadherin (P=0.0208), N-cadherin (P=0.0038) and S100A4 (P=0.0157) was each an independent and a significant prognostic factor. We also demonstrated that cadherin switch was independently associated with poor prognosis (P=0.0143) in patients with EHCC. These results may provide novel information for selection of patients with EHCC who require adjuvant therapy and strict surveillance.
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影响因子：
37.3
作者：
Shindo K;Aishima S;Ohuchida K;Fujiwara K;Fujino M;Mizuuchi Y;Hattori M;Mizumoto K;Tanaka M;Oda Y
通讯作者：
Oda Y
影响因子：
3.3
作者：
Ryu, Han Suk;Park, Do Joong;Lee, Hye Seung
通讯作者：
Lee, Hye Seung
影响因子：
4.2
作者：
Jaeger, Tobias;Becker, Markus;Szarvas, Tibor
通讯作者：
Szarvas, Tibor
影响因子：
8.8
作者：
通讯作者：
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影响因子：
8.8
作者：
通讯作者：
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