Depauperate genetic variability detected in the American and European bison using genomic techniques.
Depauperate genetic variability detected in the American and European bison using genomic techniques.
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DOI：
10.1186/1745-6150-4-48
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发表时间：
2009-12-08
期刊：
影响因子：
5.5
通讯作者：
Bendixen C
中科院分区：
文献类型：
--
作者：
Pertoldi C;Tokarska M;Wójcik JM;Demontis D;Loeschcke V;Gregersen VR;Coltman D;Wilson GA;Randi E;Hansen MM;Bendixen C
共分析了EB中的929个多态性SNPs（使用BovineSNP 50 Illumina基因分型微珠芯片筛选的54，000个SNPs中），WB和PB中分别有1，524个和1，403个多态性SNPs。EB、WB和PB最近都经历了人口规模的急剧减少。因此，他们表现出非常depaperate基因组，偏离遗传平衡和基因组组织组成的马赛克的单倍型块：区域低单倍型多样性和高水平的连锁不平衡。在EB、WB和PB中没有发现阳性或稳定选择的证据，可能反映了漂移压倒性选择。我们认为，利用全基因组筛选技术，其次是利用较便宜的技术（如VeraCode和Fluidigm EP 1），具有很大的潜力，遗传监测的人口。此外，这些技术将允许从根本上改善圈养或管理种群的育种实践，否则会受到多态性标记有限的影响。这导致1）估计个体之间的遗传关系和2）设计试图保持或减少生态相关基因和/或整个区块中的多态性的育种策略的改进的可能性。本文由以下作者审阅：Fyodor Kondrashov和Shamil Sunyaev
A total of 929 polymorphic SNPs in EB (out of 54, 000 SNPs screened using a BovineSNP50 Illumina Genotyping BeadChip), and 1, 524 and 1, 403 polymorphic SNPs in WB and PB, respectively, were analysed. EB, WB and PB have all undergone recent drastic reductions in population size. Accordingly, they exhibited extremely depauperate genomes, deviations from genetic equilibrium and a genome organization consisting of a mosaic of haplotype blocks: regions with low haplotype diversity and high levels of linkage disequilibrium. No evidence for positive or stabilizing selection was found in EB, WB and PB, likely reflecting drift overwhelming selection. We suggest that utilization of genome-wide screening technologies, followed by utilization of less expensive techniques (e.g. VeraCode and Fluidigm EP1), holds large potential for genetic monitoring of populations. Additionally, these techniques will allow radical improvements of breeding practices in captive or managed populations, otherwise hampered by the limited availability of polymorphic markers. This result in improved possibilities for 1) estimating genetic relationships among individuals and 2) designing breeding strategies which attempt to preserve or reduce polymorphism in ecologically relevant genes and/or entire blocks. This article was reviewed by: Fyodor Kondrashov and Shamil Sunyaev
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影响因子：
3.3
作者：
Khatkar, Mehar S.;Collins, Andrew;Raadsma, Herman W.
通讯作者：
Raadsma, Herman W.
影响因子：
5.5
作者：
Pertoldi, Cino;Bach, Lars A.;Loeschcke, Volker
通讯作者：
Loeschcke, Volker
影响因子：
3.8
作者：
Tokarska, M.;Marshall, T.;Bendixen, C.
通讯作者：
Bendixen, C.
影响因子：
3.1
作者：
Wilson, GA;Strobeck, C
通讯作者：
Strobeck, C
DOI：
10.1073/pnas.91.7.2757
发表时间：
1994-03-29
影响因子：
11.1
作者：
LOFTUS, RT;MACHUGH, DE;CUNNINGHAM, P
通讯作者：
CUNNINGHAM, P