Upregulation of VEGF and FGF2 in normal rat brain after experimental intraoperative radiation therapy.
Upregulation of VEGF and FGF2 in normal rat brain after experimental intraoperative radiation therapy.
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DOI：
10.3346/jkms.2004.19.6.879
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发表时间：
2004-12
影响因子：
4.5
通讯作者：
Lee HK
中科院分区：
文献类型：
--
作者：
Kim JH;Chung YG;Kim CY;Kim HK;Lee HK
血管内皮生长因子（VEGF）和成纤维细胞生长因子（FGF）2的表达在照射的大脑进行了检查，以测试如何单次高剂量辐射，类似于用于术中放射治疗给予正常大脑，可以影响血管内皮。在大鼠颅骨钻孔后，大脑半球暴露于单次10戈伊剂量的γ射线，并在照射后1、2、4、6和8周评估辐射效应。组织学改变如反应性胶质增生、炎症、血管增生和坏死与照射后时间有关。酶联免疫吸附测定法检测放射组2周和8周VEGF和FGF2的表达。免疫组化检测VEGF的表达，与假手术组比较，VEGF阳性细胞数随时间推移逐渐增多。结论：放射性损伤主要表现为放射性坏死，并伴有VEGF和FGF2的表达。提示VEGF和FGF2可能在术中大剂量照射后的放射损伤中起一定作用。
The expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)2 in the irradiated brain was examined to test how a single high dose radiation, similar to that used for intraoperative radiation therapy given to the normal cerebrum, can affect the vascular endothelium. After a burr hole trephination in the rat skull, the cerebral hemisphere was exposed to a single 10 Gy dose of gamma rays, and the radiation effect was assessed at 1, 2, 4, 6, and 8 weeks after irradiation. His-tological changes, such as reactive gliosis, inflammation, vascular proliferation and necrosis, were correlated with the duration after irradiation. Significant VEGF and FGF2 expression in the 2- and 8-week were detected by enzyme-linked immunosorbent assay quantification in the radiation group. Immunohistochemical study for VEGF was done and the number of positive cells gradually increased over time, compared with the sham operation group. In conclusion, the radiation injuries consisted of radiation necrosis associated with the expression of VEGF and FGF2. These findings indicate that VEGF and FGF2 may play a role in the radiation injuries after intraoperative single high-dose irradiation.
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