Biochemical markers for differentiation of exposures to nonplanar polychlorinated biphenyls, organochlorine pesticides, or 2,3,7, 8-tetrachlorodibenzo-p-dioxin in trout liver.
Biochemical markers for differentiation of exposures to nonplanar polychlorinated biphenyls, organochlorine pesticides, or 2,3,7, 8-tetrachlorodibenzo-p-dioxin in trout liver.
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用于区分鳟鱼肝脏中非平面多氯联苯、有机氯农药或 2,3,7,8-四氯二苯并-对二恶英暴露的生化标记。
DOI：
10.1006/eesa.1998.1675
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发表时间：
1998
影响因子：
6.8
通讯作者：
Zdenka Svobodovȥ
中科院分区：
文献类型：
--
作者：
Miroslav Machala;Petr Drȥbek;Jiͅȷ Neča;Jitka Kolȥͅovȥ;Zdenka Svobodovȥ
以虹鳟（Oncorhynchus mykiss）幼鱼为材料，研究了单次腹腔注射典型污染物2，2 '，4，4'，5，5 '-六氯联苯（PCB 153，50 mg/kg）、p，p'-DDE（50 mg/kg）和2，3，7，8-四氯二苯并-p-二恶英（TCDD，200 ng/kg）对幼鱼肝脏解毒酶活性的影响。发现所测试的外源性物质对微粒体细胞色素P450依赖性睾酮羟化酶活性的不同调节：PCB 153特异性诱导16 β-羟化酶活性，而p，p '-DDE降低细胞色素P4503 A依赖性6 β-羟化以及16 α-和2 α-羟化。TCDD没有调节睾酮羟化酶活性，但TCDD给药后观察到细胞色素P4501 A活性的强烈诱导;因此，细胞色素P4501 A不参与睾酮的羟化。鳟鱼肝微粒体谷胱甘肽S-转移酶（GST）的活性，增强了所有的外源性物质测试，被认为是一个敏感的非特异性的氧化应激生化标志物;细胞溶质谷胱甘肽还原酶是一个不太敏感的指标氧化应激和诱导显着只有处理与p，p '-DDE。PCB 153或p，p '-DDE可诱导胞浆GST对依他尼酸（GST-ETHA）的活性，而TCDD则不能诱导。调节肝微粒体睾酮羟化酶的活动和GST-ETHA的诱导似乎是合适的生化指标急性接触非平面多氯联苯和有机氯，不诱导虹鳟鱼细胞色素P4501 A酶，而微粒体GST和胞浆谷胱甘肽还原酶可能成为氧化应激的早期生化指标。
The effects of a single intraperitoneal dose of the prototypical contaminant nonplanar 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153, 50 mg/kg), p,p'-DDE (50 mg/kg), or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 200 ng/kg) on the activities of hepatic detoxification enzymes were examined in the liver of immature rainbow trout (Oncorhynchus mykiss). Different modulations of the tested xenobiotics on microsomal cytochrome P450-dependent testosterone hydroxylase activities were found: PCB 153 specifically induced 16beta-hydroxylase activity, whereas p,p'-DDE decreased cytochrome P4503A-dependent 6beta-hydroxylation as well as 16alpha- and 2alpha-hydroxylation. TCDD did not modulate testosterone hydroxylase activities, but a strong induction of cytochrome P4501A activity was observed after TCDD administration; hence, cytochrome P4501A is not involved in the hydroxylation of testosterone. Trout hepatic microsomal glutathione S-transferase (GST) activity, enhanced by all the xenobiotics tested, was found to be a sensitive nonspecific biochemical marker of oxidative stress; cytosolic glutathione reductase was a less sensitive indicator of oxidative stress and was induced significantly only by treatment with p,p'-DDE. Cytosolic GST activity toward ethacrynic acid (GST-ETHA) was induced by PCB 153 or p,p'-DDE, but not by TCDD. Modulations of hepatic microsomal testosterone hydroxylase activities and induction of GST-ETHA appeared to be suitable biochemical markers of acute exposure to nonplanar PCBs and organochlorines that do not induce cytochrome P4501A enzymes in rainbow trout, whereas microsomal GST and cytosolic glutathione reductase may become early biochemical indicators of oxidative stress.
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还原氧激活大鼠肝微粒体谷胱甘肽 S-转移酶。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Aniya,Y;Anders,MW
通讯作者：
Anders,MW
影响因子：
10.4
作者：
通讯作者：
--
邻位和非邻位取代的多氯联苯同系物对 scup（Stenotomus chrysops）肝单加氧酶系统的影响。
影响因子：
3.8
作者：
Gooch,JW;Elskus,AA;Kloepper-Sams,PJ;Hahn,ME;Stegeman,JJ
通讯作者：
Stegeman,JJ
影响因子：
5.8
作者：
HAASCH, ML;GRAF, WK;LECH, JJ
通讯作者：
LECH, JJ