Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats.
Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats.
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重复电针治疗通过抑制慢性神经病理性疼痛大鼠的脊髓胶质细胞活化来减轻痛觉过敏
DOI：
10.1186/s12906-018-2134-8
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发表时间：
2018-02-21
影响因子：
--
通讯作者：
Liu JL
中科院分区：
文献类型：
--
作者：
Wang JY;Gao YH;Qiao LN;Zhang JL;Duan-Mu CL;Yan YX;Chen SP;Liu JL
研究背景已有证据表明，脊髓内的神经胶质细胞在慢性神经病理性疼痛的发生发展中起着重要作用，在电针干预的镇痛效应中也起着复杂的作用。但脊髓小胶质细胞和星形胶质细胞在电针镇痛过程中的作用尚不清楚。采用坐骨神经慢性压迫性损伤(CCI)建立神经病理性疼痛模型。将大鼠随机分为假手术组、 + 电针组、 + 电针组。电针双侧足三里-炎陵泉。测定双侧后爪的机械痛阈值(时间反应和力反应)和热痛阈值(PTS)。免疫荧光染色检测腰髓背角(L4~5)小胶质细胞数量和星形胶质细胞活性，Western blotting检测胶质纤维酸性蛋白(GFAP)蛋白的表达。结果CCI后，同侧后爪力学和热应力从术后第3天开始显著降低(P<； )，对侧后爪力学PT从术后第6天开始显著降低(P<； 0.05)。大鼠双侧背根突浅板层Iba-1标记的小胶质细胞数和胶质纤维酸性蛋白标记的星形胶质细胞的荧光强度也显著增加(P<； )。重复电针后，双侧后足的力学和温度PTS均有显著改善(P<； )。电针干预2天可显著抑制小胶质细胞数量的增加，电针干预2周可抑制小胶质细胞的平均荧光强度。结论重复电针可减轻慢性神经病理性疼痛大鼠的神经病理性疼痛和镜像性疼痛，其机制可能与其下调腰髓胶质细胞、小胶质细胞和星形胶质细胞的活化有关(P<； 0.0 5)。
BackgroundCumulated evidence reveals that glial cells in the spinal cord play an important role in the development of chronic neuropathic pain and are also complicated in the analgesic effect of EA intervention. But the roles of microgliacytes and astrocytes of spinal cord in the process of EA analgesia remain unknown.MethodsA total of 120 male Wistar rats were used in the present study. The neuropathic pain model was established by chronic constrictive injury (CCI) of the sciatic nerve. The rats were randomly divided into sham group, CCI group, and sham CCI + EA group, and CCI + EA group. EA was applied to bilateral Zusanli (ST36)-Yanlingquan (GB34). The mechanical (both time and force responses) and thermal pain thresholds (PTs) of the bilateral hind-paws were measured. The number of microgliacytes and activity of astrocytes in the dorsal horns (DHs) of lumbar spinal cord (L4–5) were examined by immunofluorescence staining, and the expression of glial fibrillary acidic protein (GFAP) protein was detected by western blot.ResultsFollowing CCI, both mechanical and thermal PTs of the ipsilateral hind-paw were significantly decreased beginning from the 3rd day after surgery (P< 0.05), and the mechanical PT of the contralateral hind-paw was considerably decreased from the 6th day on after surgery (P< 0.05). CCI also significantly upregulated the number of Iba-1 labeled microgliacytes and the fluorescence intensity of glial fibrillary acidic protein (GFAP) -labeled astrocyte in the superficial laminae of DHs on bilateral sides (P< 0.05). After repeated EA, the mechanical and thermal PTs at bilateral hind-paws were significantly relieved (P< 0.05). The increased of number of microgliacytes was markedly suppressed by 2 days’ EA intervention, and the average fluorescence intensity was suppressed by 2 weeks’ EA. The expression of GFAP protein were down-regulated by 1 and 2 weeks’ EA treatment, respectively (P< 0.05).ConclusionsRepeated EA can relieve neuropathic pain and mirror-image pain in chronic neuropathic pain rats, which is probably associated with its effect in downregulating glial cell activation of the lumbar spinal cord, the microgliacyte first and astrocyte later.
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影响因子：
7.4
作者：
Choi, Hoon-Seong;Roh, Dae-Hyun;Lee, Jang-Herh
通讯作者：
Lee, Jang-Herh
影响因子：
2.5
作者：
Michot, Benoit;Deumens, Ronald;Hermans, Emmanuel
通讯作者：
Hermans, Emmanuel
影响因子：
6.3
作者：
Hansson, E.
通讯作者：
Hansson, E.
影响因子：
7.4
作者：
BENNETT, GJ;XIE, YK
通讯作者：
XIE, YK
DOI：
10.1097/aco.0b013e32830edbdf
发表时间：
2008-10
期刊：
Current opinion in anaesthesiology
影响因子：
--
作者：
Ren K;Dubner R
通讯作者：
Dubner R