Immunogenicity analysis of genetically conserved segments in Plasmodium ovale merozoite surface protein-8
Immunogenicity analysis of genetically conserved segments in Plasmodium ovale merozoite surface protein-8
复制标题
卵形疟原虫裂殖子表面蛋白8遗传保守片段的免疫原性分析
DOI：
10.1186/s13071-019-3412-0
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Yang Cheng
中科院分区：
文献类型：
--
作者：
XinXin Zhang;Ruilin Chu;Sui Xu;Haitian Fu;Jianxia Tang;Limei Chen;Xiaodan Shi;Jing Chen;Yuhong Li;Guoding Zhu;Eun-Taek Han;Yinghua Xuan;Jun Cao;Yang Cheng
Background:Plasmodium ovale is widely distributed across tropical regions and has two closely related but distinct species, namely P. ovale curtisi and P. ovale wallikeri. Combining genetic characterization with the immunogenicity of merozoite surface protein-8 (MSP-8) supports considering MSP-8 as a candidate target for blood-stage vaccines against malaria. However, no previous studies have focused on characterizing the genetic diversity and immunogenicity of PoMSP-8.Methods:Blood samples were collected from 42 patients infected with P. ovale. The patients were migrant workers returning to the Jiangsu Province from Africa; genomic DNA was extracted from their blood samples for sequencing and protein expression. The recombinant PoMSP-8 (rPoMSP-8) proteins were expressed and purified to assess their immune responses in BALB/c mice.Results:The sequences of the P. ovale curtisi and P. ovale wallikeri msp8 genes were completely conserved in each isolate. The rPoMSP-8 proteins were successfully expressed and purified as ~70 kDa proteins. Antibodies raised against rPoMSP-8 in mice showed appropriate immunoreactivity, as evidenced by immunoblotting. These specific antibodies were detected at day 7 post-immunization, and their levels increased throughout the whole immunization period. rPoMSP-8-raised antibodies had high endpoint titers (1:5,120,000) and high avidity (PocMSP-8: 94.84%, PowMSP-8: 92.69%). Cross-reactivity between rPocMSP-8 and rPowMSP-8 was observed with each anti-PoMSP8-specific antibody.Conclusions:Remarkable conservation and high immunogenicity was observed in both rPoMSP-8s. Intriguingly, cross-reaction between rPocMSP-8 and rPowMSP-8 was detected, suggesting that a single PoMSP8-based construction might be applicable for both species.
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影响因子：
2.2
作者：
Osier FH;Polley SD;Mwangi T;Lowe B;Conway DJ;Marsh K
通讯作者：
Marsh K
影响因子：
82.9
作者：
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影响因子：
3.1
作者：
Franks, S;Baton, L;Riley, EM
通讯作者：
Riley, EM
设计和监测针对多种抗原的疟疾疫苗的策略。
影响因子：
7.3
作者：
Barry AE;Arnott A
通讯作者：
Arnott A
从非洲进口到中国的卵形疟原虫和 P. ovale wallikeri 裂殖子表面蛋白 1 (MSP-1) N 端遗传多样性有限
影响因子：
3.2
作者：
Chu R;Zhang X;Xu S;Chen L;Tang J;Li Y;Chen J;Xuan Y;Zhu G;Cao J;Cheng Y
通讯作者：
Cheng Y