Limited genetic diversity of N-terminal of merozoite surface protein-1 (MSP-1) in Plasmodium ovale curtisi and P. ovale wallikeri imported from Africa to China.
Limited genetic diversity of N-terminal of merozoite surface protein-1 (MSP-1) in Plasmodium ovale curtisi and P. ovale wallikeri imported from Africa to China.
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从非洲进口到中国的卵形疟原虫和 P. ovale wallikeri 裂殖子表面蛋白 1 (MSP-1) N 端遗传多样性有限
DOI：
10.1186/s13071-018-3174-0
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发表时间：
2018-11-16
影响因子：
3.2
通讯作者：
Cheng Y
中科院分区：
文献类型：
--
作者：
Chu R;Zhang X;Xu S;Chen L;Tang J;Li Y;Chen J;Xuan Y;Zhu G;Cao J;Cheng Y
疟原虫裂殖子表面蛋白-1（MSP-1）在裂殖子侵入过程中释放到血流中，因此代表了关键的疟疾疫苗靶标。虽然大量的研究工作已经致力于揭示恶性疟原虫和间日疟原虫MSP-1的遗传多样性，但关于卵形疟原虫的遗传谱和结构的信息很少。因此，本研究的目的是通过在核苷酸和蛋白质水平上表征MSP-1 N-末端序列来确定卵圆藻两个亚种之间的遗传变异程度。应用聚合酶链反应（PCR）技术对126例输入性疟疾患者的MSP-1基因N端进行扩增。然后使用GeneDoc、MegAlign、MEGA 7和DnaSP v.6程序对PCR产物进行测序和分析。卵圆毕赤酵母和卵圆毕赤酵母MSP-1基因（pomsp 1）的平均成对核苷酸差异度（π）分别为0.01043和0.01974，单倍型多样度（Hd）分别为0.746和0.598。检测到的大多数核苷酸替换是非同义的，这表明pomsp 1的遗传变异是通过积极的多样化选择来维持的，从而表明它们作为保护性免疫反应的潜在靶点的作用。卵圆毕赤酵母和卵圆毕赤酵母MSP-1的氨基酸取代可分别归类为五种和三种独特的氨基酸变体。江苏省输入性疟疾病例pomsp 1基因突变多样性较低，免疫原性和功能分析有待进一步研究。本文的在线版本（10.1186/s13071-018-3174-0）包含补充材料，可供授权用户使用。
Plasmodium merozoite surface protein-1 (MSP-1) is released into the bloodstream during merozoite invasion, and thus represents a crucial malarial vaccine target. Although substantial research effort has been devoted to uncovering the genetic diversity of MSP-1 for P. falciparum and P. vivax, there is minimal information available regarding the genetic profiles and structure of P. ovale. Therefore, the aim of the present study was to determine the extent of genetic variation among two subspecies of P. ovale by characterizing the MSP-1 N-terminal sequence at the nucleotide and protein levels. N-terminal of MSP-1 gene were amplified from 126 clinical samples collected from imported cases of malaria in migrant workers returning to Jiangsu Province from Africa using a conventional polymerase chain reaction (PCR) assay. The PCR products were then sequenced and analyzed using the GeneDoc, MegAlign, MEGA7 and DnaSP v.6 programs. The average pairwise nucleotide diversities (π) of P. ovale curtisi and P. ovale wallikeri MSP-1 genes (pomsp1) were 0.01043 and 0.01974, respectively, and the haplotype diversity (Hd) were 0.746 and 0.598, respectively. Most of the nucleotide substitutions detected were non-synonymous, indicating that the genetic variations of pomsp1 were maintained by positive diversifying selection, thereby suggesting their role as a potential target of a protective immune response. Amino acid substitutions of P. ovale curtisi and P. ovale wallikeri MSP-1 could be categorized into five and three unique amino acid variants, respectively. Low mutational diversity was observed in pomsp1 from the Jiangsu Province imported malaria cases; further studies will be developed such as immunogenicity and functional analysis. The online version of this article (10.1186/s13071-018-3174-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1086/597414
发表时间：
2009-04-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Putaporntip C;Hongsrimuang T;Seethamchai S;Kobasa T;Limkittikul K;Cui L;Jongwutiwes S
通讯作者：
Jongwutiwes S
影响因子：
3
作者：
Bichara C;Flahaut P;Costa D;Bienvenu AL;Picot S;Gargala G
通讯作者：
Gargala G
影响因子：
6.4
作者：
Egan, AF;Morris, J;Riley, EM
通讯作者：
Riley, EM
影响因子：
2.8
作者：
HOLDER, AA;BLACKMAN, MJ;SHAI, S
通讯作者：
SHAI, S
影响因子：
3
作者：
Bharti PK;Shukla MM;Sharma YD;Singh N
通讯作者：
Singh N