The effect of ultrasound stimulation on the cytoskeletal organization of chondrocytes seeded in three-dimensional matrices.
The effect of ultrasound stimulation on the cytoskeletal organization of chondrocytes seeded in three-dimensional matrices.
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DOI：
10.1159/000339772
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Subramanian A
中科院分区：
文献类型：
--
作者：
Noriega S;Hasanova G;Subramanian A
评估了低强度扩散超声（LIDUS）刺激对接种在三维支架中的软骨细胞细胞骨架组织的影响。将接种在三维壳聚糖基质上的软骨细胞在5.0兆赫兹（约15千帕，51秒，每天4次）的LIDUS下暴露，以研究肌动蛋白、微管蛋白和波形蛋白的组织情况。结果显示，肌动蛋白呈现胞浆点状分布，微管蛋白呈现微管近乎平行的组织形式，而波形蛋白的分布未受影响。接种在三维支架上的软骨细胞通过肌动蛋白应力纤维的断裂对超声刺激作出反应，并且对ROCK抑制剂（Y27632）的存在敏感。在超声作用下，应力纤维形成的关键要素ROCK - I以及mDia1的基因表达显著上调。我们得出结论，细胞骨架分析和基因表达的结果都支持这样的观点：超声刺激下出现点状肌动蛋白的同时伴随着RhoA/ROCK通路的上调。
The impact of low intensity diffuse ultrasound (LIDUS) stimulation on the cytoskeletal organization of chondrocytes seeded in 3D scaffolds was evaluated. Chondrocytes seeded on 3D chitosan matrices were exposed to LIDUS at 5.0 MHz (~15kPa, 51-secs, 4-applications/day) in order to study the organization of actin, tubulin and vimentin. The results showed that actin presented a cytosolic punctuated distribution, tubulin presented a quasi parallel organization of microtubules whereas vimentin distribution was unaffected. Chondrocytes seeded on 3D scaffolds responded to US stimulation by the disruption of actin stress fibers and were sensitive to the presence of ROCK inhibitor (Y27632). The gene expression of ROCK-I, a key element in the formation of stress fibers and mDia1, was significantly up-regulated under the application of US. We conclude that the results of both the cytoskeletal analyses and gene expression support the argument that the presence of punctuated actin upon US stimulation was accompanied by the up-regulation of the RhoA/ROCK pathway.
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DOI：
10.1083/jcb.200301147
发表时间：
2003-06-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Janson ME;de Dood ME;Dogterom M
通讯作者：
Dogterom M
影响因子：
--
作者：
Darenfed, Hassina;Dayanandan, Bama;Mandato, Craig A.
通讯作者：
Mandato, Craig A.
影响因子：
2.8
作者：
Parvizi, J;Wu, CC;Bolander, ME
通讯作者：
Bolander, ME
影响因子：
2.4
作者：
Knight, MM;Toyoda, T;Bader, DL
通讯作者：
Bader, DL
DOI：
10.1083/jcb.153.6.1175
发表时间：
2001-06-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Riveline D;Zamir E;Balaban NQ;Schwarz US;Ishizaki T;Narumiya S;Kam Z;Geiger B;Bershadsky AD
通讯作者：
Bershadsky AD