A New Approach for the AFM-Based Mechanical Characterization of Biological Samples.
A New Approach for the AFM-Based Mechanical Characterization of Biological Samples.
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DOI：
10.1155/2020/2896792
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Stylianou A
中科院分区：
文献类型：
--
作者：
Kontomaris SV;Malamou A;Stylianou A
原子力显微镜纳米压痕技术是在纳米尺度上对生物样品进行力学表征的有力工具。实验结果的数据分析通常采用赫兹接触力学进行。然而，前述理论仅适用于样品为均匀各向同性且呈现线弹性响应的情况。然而，生物样品通常表现出与深度相关的力学特性，因此不能使用赫兹分析。因此，在本文中，提出了一种不同的方法，基于一种新的物理量，用于确定纳米级的机械性能。上述物理量是压头单位体积所做的功。上述分析的优点是显著的，因为上述幅度可以用来检验样本是否可以近似为弹性半空间。如果这种近似是有效的，那么新提出的方法能够准确地计算杨氏模数。此外，它还可以用来探索具有随深度变化的力学行为的样品的机械性能。总之，提出的分析提供了一种准确而简单的技术，可以在纳米尺度上确定生物样品的机械性能，也可以在赫兹极限之外使用。
The AFM nanoindentation technique is a powerful tool for the mechanical characterization of biological samples at the nanoscale. The data analysis of the experimentally obtained results is usually performed using the Hertzian contact mechanics. However, the aforementioned theory can be applied only in cases that the sample is homogeneous and isotropic and presents a linear elastic response. However, biological samples often present depth-dependent mechanical properties, and the Hertzian analysis cannot be used. Thus, in this paper, a different approach is presented, based on a new physical quantity used for the determination of the mechanical properties at the nanoscale. The aforementioned physical quantity is the work done by the indenter per unit volume. The advantages of the presented analysis are significant since the abovementioned magnitude can be used to examine if a sample can be approximated to an elastic half-space. If this approximation is valid, then the new proposed method enables the accurate calculation of Young's modulus. Additionally, it can be used to explore the mechanical properties of samples that are characterized by a depth-dependent mechanical behavior. In conclusion, the proposed analysis presents an accurate yet simple technique for the determination of the mechanical properties of biological samples at the nanoscale that can be also used beyond the Hertzian limit.
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