Expression of exogenous c‐myc oncogene does not initiate DNA synthesis in primary rat hepatocyte cultures
Expression of exogenous c‐myc oncogene does not initiate DNA synthesis in primary rat hepatocyte cultures
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外源性 c-myc 癌基因的表达不会在原代大鼠肝细胞培养物中启动 DNA 合成
DOI：
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发表时间：
1992
影响因子：
5.6
通讯作者：
M. Kaser
中科院分区：
文献类型：
--
作者：
G. Skouteris;M. Kaser
Cultured hepatocytes from adult rats stimulated with combinations of growth factors enter into S phase but do not undergo multiple rounds of DNA synthesis nor mitosis. We have examined the potential of an introduced oncogene to induce alterations in the DNA synthetic activity of the cultured hepatocytes in response to epidermal growth factor (EGF). Overexpression of c‐myc did not initiate significant DNA synthesis in rat hepatocyte cultures alone, although it cooperated with added EGF to super‐induce thymidine incorporation into DNA. From our results, it is suggested that EGF is also necessary to initiate hepatocyte DNA synthesis probably by inducing a battery of cell cycle‐related genes if incubated with c‐myc transfected cultures for only 5 hours. Hepatocyte polypeptides reacting with antiMYC antisera were found to migrate between 55–67 KDa in SDS‐PAGE; only the 64–67 KDa species were found to be phosphorylated, and the observed size heterogeneity may be due to proteolytic degradation or may reflect presently unknown posttranslational modifications.
影响因子：
56.9
作者：
T. Stewart;A. Bellvé;P. Leder
通讯作者：
T. Stewart;A. Bellvé;P. Leder
通过用猿猴病毒 40 DNA 转染来转化大鼠肝细胞，以产生增殖的分化细胞。
影响因子：
11.2
作者：
Woodworth,C;Secott,T;Isom,HC
通讯作者：
Isom,HC