Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus.
Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus.
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DOI：
10.1016/j.nbd.2010.07.018
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发表时间：
2010-12
影响因子：
6.1
通讯作者：
Wilcox, K. S.
中科院分区：
文献类型：
--
作者：
Takahashi, D. K.;Vargas, J. R.;Wilcox, K. S.
在人类和动物的颞叶癫痫(TLE)模型中，普遍描述了伴发神经细胞丢失的深部星形胶质细胞增多症。在红藻氨酸诱导的TLE癫痫持续状态(SE)模型中，海马区星形胶质细胞在SE后不久和自发性癫痫发作之前就开始反应。为了确定海马区星形胶质细胞在SE后不久是否表现出功能变化，我们使用全细胞贴片技术记录了SR101标记的星形胶质细胞在对照组和海人酸组大鼠的海马脑片上。谷氨酸转运体依赖的电流具有明显更快的衰减时间动力学，此外，星形胶质细胞之间的染料偶联显著增加。与反应性星形胶质细胞的染料偶联增加一致，免疫印迹实验显示胶质纤维酸性蛋白(GFAP)和连接蛋白43(由星形胶质细胞表达的主要缝隙连接蛋白)均显著增加。与在难治性癫痫患者的切除组织中观察到的相反，在KA诱导SE后不久没有观察到钾电流的变化。虽然在这种TLE模型中，在低发作概率的最初阶段已经发现了神经元功能的许多变化，但本研究有助于越来越多的文献表明星形胶质细胞在癫痫发生过程中发挥作用。
Profound astrogliosis coincident with neuronal cell loss is universally described in human and animal models of temporal lobe epilepsy (TLE). In the kainic acid-induced status epilepticus (SE) model of TLE, astrocytes in the hippocampus become reactive soon after SE and before the onset of spontaneous seizures. To determine if astrocytes in the hippocampus exhibit changes in function soon after SE, we recorded from SR101-labeled astrocytes using the whole-cell patch technique in hippocampal brain slices prepared from control and kainic-acid treated rats. Glutamate transporter-dependent currents were found to have significantly faster decay time kinetics and in addition, dye coupling between astrocytes was substantially increased. Consistent with an increase in dye coupling in reactive astrocytes, immunoblot experiments demonstrated a significant increase in both glial fibrillary acidic protein (GFAP) and connexin 43, a major gap junction protein expressed by astrocytes. In contrast to what has been observed in resected tissue from patients with refractory epilepsy, changes in potassium currents were not observed shortly after KA-induced SE. While many changes in neuronal function have been identified during the initial period of low seizure probability in this model of TLE, the present study contributes to the growing body of literature suggesting a role for astrocytes in the process of epileptogenesis.
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影响因子：
5.3
作者：
Djukic, Biljana;Casper, Kristen B.;McCarthy, Ken D.
通讯作者：
McCarthy, Ken D.
影响因子：
2.5
作者：
Diamond, JS;Jahr, CE
通讯作者：
Jahr, CE
影响因子：
5.3
作者：
Haber, Michael;Zhou, Lei;Murai, Keith K.
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Murai, Keith K.
影响因子：
56.9
作者：
Ge, Woo-Ping;Yang, Xiu-Juan;Duan, Shumin
通讯作者：
Duan, Shumin
影响因子：
5.3
作者：
Diamond, JS
通讯作者：
Diamond, JS