New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis.
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis.
复制标题
DOI：
10.1186/1471-2164-14-175
复制
发表时间：
2013-03-14
期刊：
影响因子：
4.4
通讯作者：
Zaha A
中科院分区：
文献类型：
--
作者：
Siqueira FM;Thompson CE;Virginio VG;Gonchoroski T;Reolon L;Almeida LG;da Fonsêca MM;de Souza R;Prosdocimi F;Schrank IS;Ferreira HB;de Vasconcelos AT;Zaha A
猪肺炎支原体、絮状支原体和猪鼻支原体生活在猪呼吸道中。M. flocculare是一种共生细菌，与M.猪肺炎是地方性猪肺炎的病原体。M.猪鼻炎也是致病性的，引起多浆膜炎和关节炎。在这项工作中，我们提出的基因组序列的M。flocculare和M.猪肺炎支原体菌株7422的基因组，并与其他M.猪肺炎支原体和M.猪鼻鱼基因组进行这些分析以确定可能有助于解释这些种属在猪呼吸道中的不同行为的可能特征。分析了三个物种的整体基因组组织，揭示了ORF簇（OC）差异很大，倒位和重排是常见的。虽然M. flocculare和M.虽然在基因内容方面，基因组DNA显示出高度的相似性，但只有一些基因组区域显示出相当大的同线性。在M.和M.在基因组结构和组织上显示出差异。P97家族的一些编码粘附素的基因在M.一些含有序列差异或缺乏被认为对粘附宿主细胞重要的结构域。通过系统发育分析，证实了这三个物种的系统发育关系。参与代谢的基因组，特别是在核酸合成和核苷酸代谢的前体的摄取中，在三个物种中的拷贝数和存在/不存在方面显示出一些差异。通过对三种猪呼吸道支原体的比较分析，发现了可能与它们不同行为有关的一些特征。M.和M. flocculare显示出许多差异，这可能有助于解释为什么一个物种是致病性的，而另一个被认为是致病性的。然而，这是不可能的，以确定特定的毒力决定因素，可以解释的差异，分析物种的致病性。分枝猪鼻鱼基因组中涉及代谢和逃避宿主免疫系统的某些成分存在差异，这可能有助于其生长侵略性。鉴定了几个水平基因转移事件。基因组分析将M. hypopalanum，M. flocculare和M.猪鼻属的一个分支。
Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts. The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species. The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host’s immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade.
登录
查看更多内容
影响因子：
2.8
作者：
Boye, M;Jensen, TK;Friis, NF
通讯作者：
Friis, NF
影响因子：
3.6
作者：
Burnett, TA;Dinkla, K;Djordjevic, SP
通讯作者：
Djordjevic, SP
影响因子：
2.1
作者：
Bizarro, Cristiano Valim;Schuck, Desirée Cigaran
通讯作者：
Schuck, Desirée Cigaran
影响因子：
3.6
作者：
Deutscher, Ania T.;Jenkins, Cheryl;Djordjevic, Steven P.
通讯作者：
Djordjevic, Steven P.
影响因子：
3.3
作者：
de Castro, Luiza Amaral;Pedroso, Thiago Rodrigues;Ferreira, Henrique Bunselmeyer
通讯作者：
Ferreira, Henrique Bunselmeyer