Composition of dietary fat source shapes gut microbiota architecture and alters host inflammatory mediators in mouse adipose tissue.
Composition of dietary fat source shapes gut microbiota architecture and alters host inflammatory mediators in mouse adipose tissue.
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饮食脂肪源的组成形状形状肠道菌群结构和改变小鼠脂肪组织中的炎症介质。
DOI：
10.1177/0148607113486931
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Chang EB
中科院分区：
文献类型：
--
作者：
Huang EY;Leone VA;Devkota S;Wang Y;Brady MJ;Chang EB
越来越多的证据表明，饮食因素可以极大地改变肠道微生物组，从而导致代谢紊乱和肥胖的进展。在这方面，肠系膜脂肪组织已被牵连在介导这些过程中，通过制定促炎性脂肪因子。在这项研究中，我们通过确定膳食脂肪含量和来源对肠道微生物群的影响，以及对肠系膜和外周脂肪细胞的脂肪因子谱的影响，研究了这些事件之间的关系。成年雄性C57 B1/6小鼠饲喂基于乳脂、猪油（SFA来源）或红花油（PUFA）的高脂肪饮食四周。测量体重和摄食量。分离粪便16 S rRNA并通过T-RFLP进行分析，以及通过下一代测序进行可变V3-4序列标签分析。通过qRT-PCR分析肠系膜和性腺脂肪样品的脂肪生成和炎症介质。高脂肪喂养引起更多的体重增加，伴随着热量消耗的增加，相对于低脂饮食。此外，每种高脂饮食诱导显著和特异性的16 S rRNA促性腺激素生成谱，其与肠系膜和性腺脂肪库的不同炎症和脂肪生成介质谱相关。我们的研究结果支持了这样的观点：膳食脂肪组成既可以重塑肠道微生物群，也可以改变宿主脂肪组织的炎症/脂肪生成特征。他们还证明了膳食脂肪来源、肠道微生物群和肠系膜脂肪的炎症特征之间的相互依赖性，这些因素可以共同影响宿主的代谢状态。
Growing evidence shows that dietary factors can dramatically alter the gut microbiome in ways that contribute to metabolic disturbance and progression of obesity. In this regard, mesenteric adipose tissue has been implicated in mediating these processes through the elaboration of pro-inflammatory adipokines. In this study, we examined the relationship of these events by determining the effects of dietary fat content and source on gut microbiota, as well as the effects on adipokine profiles of mesenteric and peripheral adipocytes. Adult male C57Bl/6 mice were fed milk fat-, lard-(SFA sources), or safflower oil (PUFA)- based high fat diets for four weeks. Body mass and food consumption were measured. Stool 16S rRNA was isolated and analyzed via T-RFLP as well as variable V3-4 sequence tags via next gen sequencing. Mesenteric and gonadal adipose samples were analyzed for both lipogenic and inflammatory mediators via qRT-PCR. High-fat feedings caused more weight gain with concomitant increases in caloric consumption relative to low-fat diets. Additionally, each of the high fat diets induced dramatic and specific 16S rRNA phylogenic profiles that were associated with different inflammatory and lipogenic mediator profile of mesenteric and gonadal fat depots. Our findings support the notion that dietary fat composition can both reshape the gut microbiota as well as alter host adipose tissue inflammatory/lipogenic profiles. They also demonstrate the interdependency of dietary fat source, commensal gut microbiota, and inflammatory profile of mesenteric fat that can collectively impact the host metabolic state.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
29
作者：
Chalkiadaki A;Guarente L
通讯作者：
Guarente L
影响因子：
64.8
作者：
Devkota, Suzanne;Wang, Yunwei;Musch, Mark W.;Leone, Vanessa;Fehlner-Peach, Hannah;Nadimpalli, Anuradha;Antonopoulos, Dionysios A.;Jabri, Bana;Chang, Eugene B.
通讯作者：
Chang, Eugene B.
影响因子：
7.1
作者：
Djousse, Luc;Gaziano, J. Michael;Lee, I-Min
通讯作者：
Lee, I-Min